Accumulation of DSBs in {gamma}-H2AX domains fuel chromosomal aberrations
- Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Institute of Molecular Radiation Biology, Ingolstaedter Landstrasse 1, 85764 Neuherberg (Germany)
- Bundeswehr Institute of Radiobiology, Neuherbergstrasse, 11, D-80937 Munich (Germany)
DNA double strand breaks (DSBs) pose a severe hazard to the genome as erroneous rejoining of DSBs can lead to mutation and cancer. Here, we have investigated the correlation between X irradiation-induced {gamma}-H2AX foci, theoretically induced DSBs, and the minimal number of mis-rejoined DNA breaks (MNB) in irradiated lymphocytes obtained from two healthy humans by painting of the whole chromosome complement by spectral karyotyping. There were less {gamma}-H2AX foci/dose than theoretically expected, while misrepair, as expressed by MNB/{gamma}-H2AX focus, was similar at 0.5 and 1 Gy but 3.6-fold up at 3 Gy. Hence, our results suggest that X-ray-induced {gamma}-H2AX foci in G0 lymphocyte nuclei contain more than one DSB and that the increasing number of DSBs per {gamma}-H2AX repair factory lead to an increased rate of misrepair.
- OSTI ID:
- 21143753
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 371, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.04.127; PII: S0006-291X(08)00805-X; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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