Quantitative expression patterns of peroxisome proliferator-activated receptor-{beta}/{delta} (PPAR{beta}/{delta}) protein in mice

Journal Article · Fri Jul 04 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.04.086· OSTI ID:21143742

Girroir, Elizabeth E ^[1]; Hollingshead, Holly E ^[1]; Pengfei, He; Bokai, Zhu; Perdew, Gary H ^[1]; Peters, Jeffrey M ^[1]

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1. Department of Veterinary and Biomedical Sciences, Center of Molecular Toxicology and Carcinogenesis, Pennsylvania State University, 312 Life Sciences Building, University Park, PA 16802 (United States)

The expression patterns of PPAR{beta}/{delta} have been described, but the majority of these data are based on mRNA data. To date, there are no reports that have quantitatively examined the expression of PPAR{beta}/{delta} protein in mouse tissues. In the present study, a highly specific PPAR{beta}/{delta} antibody was developed, characterized, and used to examine tissue expression patterns of PPAR{beta}/{delta}. As compared to commercially available anti-PPAR{beta}/{delta} antibodies, one of six polyclonal anti-PPAR{beta}/{delta} antibodies developed was significantly more effective for immunoprecipitation of in vitro-translated PPAR{beta}/{delta}. This antibody was used for quantitative Western blot analysis using radioactive detection methods. Expression of PPAR{beta}/{delta} was highest in colon, small intestine, liver, and keratinocytes as compared to other tissues including heart, spleen, skeletal muscle, lung, brain, and thymus. Interestingly, PPAR{beta}/{delta} expression was localized in the nucleus and RXR{alpha} can be co-immunoprecipitated with nuclear PPAR{beta}/{delta}. Results from these studies demonstrate that PPAR{beta}/{delta} expression is highest in intestinal epithelium, liver, and keratinocytes, consistent with significant biological roles in these tissues.

• OSTI ID: 21143742
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 371, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2008.04.086; PII: S0006-291X(08)00779-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
BIOCHEMISTRY
BRAIN
EPITHELIUM
HEART
IN VITRO
LARGE INTESTINE
LIVER
LUNGS
MICE
MUSCLES
RECEPTORS
SMALL INTESTINE
SPLEEN
THYMUS