Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1

Journal Article · Fri Jun 27 04:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.04.068· OSTI ID:21143733

Moon, Jai-Hee; Yoon, Byung Sun; Kim, Bona ^[1]; Park, Gyuman ^[2]; Jung, Hye-Youn; Maeng, Isaac ^[1]; Jun, Eun Kyoung ^[1]; Yoo, Seung Jun ^[1]; Kim, Aeree ^[3]; Oh, Sejong ^[4]; Whang, Kwang Youn; Kim, Hyunggee ^[1]; Kim, Dong-Wook ^[5]; Kim, Ki Dong ^[6]; You, Seungkwon ^[1]

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1. Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, 5-1, Anam-Dong, Sungbuk-ku, Seoul 136-701 (Korea, Republic of)
2. Department of Dermatology, College of Medicine, Korea University, Seoul (Korea, Republic of)
3. Department of Pathology, College of Medicine, Korea University, Seoul (Korea, Republic of)
4. Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju (Korea, Republic of)
5. Department of Physiology, Center for Cell Therapy, Yonsei University College of Medicine, Seoul (Korea, Republic of)
6. Imgen Co., Ltd, Suwon, Gyenggi-Do (Korea, Republic of)

Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.

• OSTI ID: 21143733
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 371; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
GENES
IN VITRO
MICE
MORPHOLOGY
NERVE CELLS
STEM CELLS