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Essential roles of caspases and their upstream regulators in rotenone-induced apoptosis

Journal Article · · Biochemical and Biophysical Research Communications
OSTI ID:21143720
 [1];  [2];  [3]; ;  [4];  [1];  [3]
  1. Graduate Institute of Veterinary Medicine, College of Biological Resources and Agriculture, National Taiwan University, Taipei, Taiwan (China)
  2. Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (China)
  3. Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan (China)
  4. Genomics Research Center, Academia Sinica, Taipei, Taiwan (China)
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In the present study, we examined whether caspases and their upstream regulators are involved in rotenone-induced cytotoxicity. Rotenone significantly inhibited the proliferation of oral cancer cell lines in a dose-dependent manner compared to normal oral mucosal fibroblasts. Flow cytometric analysis of DNA content showed that rotenone treatment induced apoptosis following G2/M arrest. Western blotting showed activation of both the caspase-8 and caspase-9 pathways, which differed from previous studies conducted in other cell types. Furthermore, p53 protein and its downstream pro-apoptotic target, Bax, were induced in SAS cells after treatment with rotenone. Rotenone-induced apoptosis was inhibited by antioxidants (glutathione, N-acetylcysteine, and tiron). In conclusion, our results demonstrate significant involvement of caspases and their upstream regulators in rotenone-induced cytotoxicity.
OSTI ID:
21143720
Journal Information:
Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 371; ISSN 0006-291X; ISSN BBRCA9
Country of Publication:
United States
Language:
English

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Related Subjects

60 APPLIED LIFE SCIENCES
ANTIOXIDANTS
APOPTOSIS
DNA
DOSES
FIBROBLASTS
GLUTATHIONE
NEOPLASMS
TIRON
TOXICITY