Identification of E2F1 as a positive transcriptional regulator for {delta}-catenin

Journal Article · Fri May 02 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.02.069· OSTI ID:21143643

Kim, Kwonseop ^[1]; Oh, Minsoo; Ki, Hyunkyoung ^[2]; Tao, Wang; Bareiss, Sonja ^[1]; Fini, M Elizabeth ^[3]; Dawei, Li ^[4]

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1. Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, 600 Moye Street, Greenville, NC 27834 (United States)
2. College of Pharmacy and Research Institute of Drug development, Chonnam National University, Gwangju 500-757 (Korea, Republic of)
3. Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL 33136 (United States)
4. Department of Pathology, Harvard Medical School, Boston, MA 20115 (United States)

{delta}-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate {delta}-catenin expression in cancer. Using a human {delta}-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect {delta}-catenin transcription. Among {beta}-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased {delta}-catenin-luciferase activities while {beta}-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of {delta}-catenin-luciferase activities induced by E2F1 but did not interact with {delta}-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of {delta}-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on {delta}-catenin expression were observed only in human cancer cells expressing abundant endogenous {delta}-catenin. These studies identify E2F1 as a positive transcriptional regulator for {delta}-catenin, but further suggest the presence of strong negative regulator(s) for {delta}-catenin in prostate cancer cells with minimal endogenous {delta}-catenin expression.

• OSTI ID: 21143643
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 369; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CARCINOMAS
CELL PROLIFERATION
LUCIFERASE
POLYMERASE CHAIN REACTION
TRANSCRIPTION