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Title: A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, in common with many other positive strand RNA viruses, encodes a nucleocapsid (N) protein which can localise not only to the cytoplasm but also to the nucleolus in virus-infected cells and cells over-expressing N protein. The dynamic trafficking of positive strand RNA virus nucleocapsid proteins and PRRSV N protein in particular between the cytoplasm and nucleolus is unknown. In this study live imaging of permissive and non-permissive cell lines, in conjunction with photo-bleaching (FRAP and FLIP), was used to investigate the trafficking of fluorescent labeled (EGFP) PRRSV-N protein. The data indicated that EGFP-PRRSV-N protein was not permanently sequestered to the nucleolus and had equivalent mobility to cellular nucleolar proteins. Further the nuclear import of N protein appeared to occur faster than nuclear export, which may account for the observed relative distribution of N protein between the cytoplasm and the nucleolus.

Authors:
;  [1]; ;  [2];  [1];  [3]
  1. Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds (United Kingdom)
  2. Nebraska Center for Virology, E249A Beadle, Lincoln (United States)
  3. (United Kingdom), E-mail: j.a.hiscox@leeds.ac.uk
Publication Date:
OSTI Identifier:
21141025
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 378; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2008.04.037; PII: S0042-6822(08)00268-7; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLEACHING; CYTOPLASM; IMAGES; PROTEINS; RNA; VIRUSES

Citation Formats

You, Jae-Hwan, Howell, Gareth, Pattnaik, Asit K., Osorio, Fernando A., Hiscox, Julian A., and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds. A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging. United States: N. p., 2008. Web. doi:10.1016/j.virol.2008.04.037.
You, Jae-Hwan, Howell, Gareth, Pattnaik, Asit K., Osorio, Fernando A., Hiscox, Julian A., & Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds. A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging. United States. doi:10.1016/j.virol.2008.04.037.
You, Jae-Hwan, Howell, Gareth, Pattnaik, Asit K., Osorio, Fernando A., Hiscox, Julian A., and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds. Fri . "A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging". United States. doi:10.1016/j.virol.2008.04.037.
@article{osti_21141025,
title = {A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on live cell imaging},
author = {You, Jae-Hwan and Howell, Gareth and Pattnaik, Asit K. and Osorio, Fernando A. and Hiscox, Julian A. and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds},
abstractNote = {Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, in common with many other positive strand RNA viruses, encodes a nucleocapsid (N) protein which can localise not only to the cytoplasm but also to the nucleolus in virus-infected cells and cells over-expressing N protein. The dynamic trafficking of positive strand RNA virus nucleocapsid proteins and PRRSV N protein in particular between the cytoplasm and nucleolus is unknown. In this study live imaging of permissive and non-permissive cell lines, in conjunction with photo-bleaching (FRAP and FLIP), was used to investigate the trafficking of fluorescent labeled (EGFP) PRRSV-N protein. The data indicated that EGFP-PRRSV-N protein was not permanently sequestered to the nucleolus and had equivalent mobility to cellular nucleolar proteins. Further the nuclear import of N protein appeared to occur faster than nuclear export, which may account for the observed relative distribution of N protein between the cytoplasm and the nucleolus.},
doi = {10.1016/j.virol.2008.04.037},
journal = {Virology},
number = 1,
volume = 378,
place = {United States},
year = {Fri Aug 15 00:00:00 EDT 2008},
month = {Fri Aug 15 00:00:00 EDT 2008}
}