Lymphocytic choriomeningitis virus uses a novel endocytic pathway for infectious entry via late endosomes

Journal Article · Fri Aug 15 04:00:00 EDT 2008 · Virology

DOI:https://doi.org/10.1016/j.virol.2008.04.046· OSTI ID:21141024

Quirin, Katharina ^[1]; Eschli, Bruno ^[2]; Scheu, Isabella; Poort, Linda ^[1]; Kartenbeck, Juergen ^[3]; Helenius, Ari ^[1]

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1. Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, CH-8093 Zurich (Switzerland)
2. Institute of Experimental Immunology, University Hospital Zurich, Schmelzbergstrasse 12, CH-8091 Zurich (Switzerland)
3. Division of Cell Biology, German Cancer Research Center (DKFZ), D-69120 Heidelberg (Germany)

The endocytic entry of lymphocytic choriomeningitis virus (LCMV) into host cells was compared to the entry of viruses known to exploit clathrin or caveolae/raft-dependent pathways. Pharmacological inhibitors, expression of pathway-specific dominant-negative constructs, and siRNA silencing of clathrin together with electron and light microscopy provided evidence that although a minority population followed a classical clathrin-mediated mechanism of entry, the majority of these enveloped RNA viruses used a novel endocytic route to late endosomes. The pathway was clathrin, dynamin-2, actin, Arf6, flotillin-1, caveolae, and lipid raft independent but required membrane cholesterol. Unaffected by perturbation of Rab5 or Rab7 and apparently without passing through Rab5/EEA1-positive early endosomes, the viruses reached late endosomes and underwent acid-induced penetration. This membrane trafficking route between the plasma membrane and late endosomes may function in the turnover of a select group of surface glycoproteins such as the dystroglycan complex, which serves as the receptor of LCMV.

• OSTI ID: 21141024
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 378; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
CHOLESTEROL
DISTURBANCES
GLYCOPROTEINS
LIPIDS
MICROSCOPY
RECEPTORS
RNA
VIRUSES