Derivation of a triple mosaic adenovirus based on modification of the minor capsid protein IX

Journal Article · Fri Aug 01 04:00:00 EDT 2008 · Virology

DOI:https://doi.org/10.1016/j.virol.2008.04.023· OSTI ID:21141019

Yizhe, Tang ^[1]; Le, Long P; Matthews, Qiana L; Tie, Han; Hongju, Wu ^[1]

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1. Division of Human Gene Therapy, Departments of Medicine, Pathology, Surgery, and Obstetrics and Gynecology, and the Gene Therapy Center, University of Alabama at Birmingham, 901 19th Street South, BMR2-502, Birmingham, AL 35294 (United States)

Adenoviral capsid protein IX (pIX) has been shown to be a potential locale to insert targeting, imaging-related and therapeutic modalities by genetic modification. Recent evidences suggested that capsid protein mosaicism could be a promising strategy for improving the utility of Ad vector. In this study, we explored a method to genetically generate triple pIX mosaic Ad serotype 5 (Ad5) displaying three types of pIX on a single virion. pIXs were modified at their carboxy termini with a Flag sequence, a hexahistidine sequence (His{sub 6}) or a monomeric red fluorescent protein (mRFP1), respectively. Western blotting analysis and fluorescence microscopy of the purified recombinant viruses indicated that all three modified pIXs were incorporated into the viral particles. Immuno-gold electron microscopy (EM) further confirmed that three types of pIX indeed co-existed on an individual virion. These results firstly validated a triple mosaic capsid configuration on pIX, and demonstrated the possibility of further radical design.

• OSTI ID: 21141019
• Journal Information: Virology, Journal Name: Virology Journal Issue: 2 Vol. 377; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADENOVIRUS
ELECTRON MICROSCOPY
FLUORESCENCE
GOLD
MOSAICISM
PROTEINS