Effects of Mikania glomerata Spreng. and Mikania laevigata Schultz Bip. ex Baker (Asteraceae) extracts on pulmonary inflammation and oxidative stress caused by acute coal dust exposure

Journal Article · Mon Dec 15 04:00:00 EST 2008 · Journal of Medicinal Food

DOI:https://doi.org/10.1089/jmf.2008.0051· OSTI ID:21126136

Freitas, T P; Silveira, P C; Rocha, L G; Rezin, G T; Rocha, J; Citadini-Zanette, V; Romao, P T; Dal-Pizzol, F; Pinho, R A; Andrade, V M; Streck, E L ^[1]

---

1. University Extremo Catarinense, Criciuma (Brazil)

Several studies have reported biological effects of Mikania glomerata and Mikania laevigata, used in Brazilian folk medicine for respiratory diseases. Pneumoconiosis is characterized by pulmonary inflammation caused by coal dust exposure. In this work, we evaluated the effect of pretreatment with M. glomerata and M. laevigata extracts (MGE and MLE, respectively) (100 mg/kg, s.c.) on inflammatory and oxidative stress parameters in lung of rats subjected to a single coal dust intratracheal instillation. Rats were pretreated for 2 weeks with saline solution, MGE, or MLE. On day 15, the animals were anesthetized, and gross mineral coal dust or saline solutions were administered directly in the lung by intratracheal instillation. Fifteen days after coal dust instillation, the animals were killed. Bronchoalveolar lavage (BAL) was obtained; total cell count and lactate dehydrogenase (LDH) activity were determined. In the lung, myeloperoxidase activity, thiobarbituric acid-reactive substances (TBARS) level, and protein carbonyl and sulfhydryl contents were evaluated. In BAL of treated animals, we verified an increased total cell count and LDH activity. MGE and MLE prevented the increase in cell count, but only MLE prevented the increase in LDH. Myeloperoxidase and TBARS levels were not affected, protein carbonylation was increased, and the protein thiol levels were decreased by acute coal dust intratracheal administration. The findings also suggest that both extracts present an important protective effect on the oxidation of thiol groups. Moreover, pretreatment with MGE and MLE also diminished lung inflammatory infiltration induced by coal dust, as assessed by histopathologic analyses.

• OSTI ID: 21126136
• Journal Information: Journal of Medicinal Food, Journal Name: Journal of Medicinal Food Journal Issue: 4 Vol. 11; ISSN 1096-620X
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
COAL
DUSTS
INHIBITION
LUNGS
PLANTS
PNEUMOCONIOSES
RATS