Investigation of the interaction of dimethyl sulfoxide with lipid membranes by small-angle neutron scattering

Journal Article · Tue May 15 04:00:00 EDT 2007 · Crystallography Reports

DOI:https://doi.org/10.1134/S1063774507030364· OSTI ID:21090900

Gordeliy, V I ^[1]

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1. Joint Institute for Nuclear Research (Russian Federation)

The influence of dimethyl sulfoxide (CH{sub 3}){sub 2}SO (DMSO) on the structure of membranes of 1,2-dimiristoyl-sn-glycero-3-phosphatidylcholine (DMPC) in an excess of a water-DMSO solvent is investigated over a wide range of DMSO molar concentrations 0.0 {<=} X{sub DMSO} {<=} 1.0 at temperatures T = 12.5 and 55 deg. C. The dependences of the repeat distance d of multilamellar membranes and the thickness d{sub b} of single vesicles on the molar concentration X{sub DMSO} in the L{sub {beta}}{sub '} gel and L{sub {alpha}} liquid-crystalline phases are determined by small-angle neutron scattering. The intermembrane distance d{sub s} is determined from the repeat distance d and the membrane thickness d{sub b}. It is shown that an increase in the molar concentration X{sub DMSO} leads to a considerable decrease in the intermembrane distance and that, at X{sub DMSO} = 0.4, the neighboring membranes are virtually in steric contact with each other. The use of the deuterated phospholipid (DMSO-D6) and the contrast variation method makes it possible, for the first time, to determine the number of DMSO molecules strongly bound to the membrane.

• OSTI ID: 21090900
• Journal Information: Crystallography Reports, Journal Name: Crystallography Reports Journal Issue: 3 Vol. 52; ISSN 1063-7745; ISSN CYSTE3
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
DMSO
LECITHINS
MEMBRANES
NEUTRON DIFFRACTION
SMALL ANGLE SCATTERING