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Title: Comparative analysis of spatial organization of laccases from Cerrena maxima and Coriolus zonatus

Abstract

Laccase (oxygen oxidoreductase, EC 1.10.3.2) belongs to the multicopper oxidase family. The main function of this enzyme is to perform electron transfer from the oxidized substrate through the mononuclear copper-containing site T1 to the oxygen molecule bound to the site T3 in the trinuclear T2/T3 cluster. The structures of two new fungal laccases from C. maxima and C. zonatus were solved on the basis of synchrotron X-ray diffraction data. Both laccases show high structural homology with laccases from other sources. The role of the carbohydrate component of laccases in structure stabilization and formation of ordered protein crystals was demonstrated. In the structures of C. maxima and C. zonatus laccases, two water channels of functional importance were found and characterized. The structural results reported in the present study characterize one of the functional states of the enzyme fixed in the crystal structure.

Authors:
; ; ;  [1];  [2]
  1. Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)
  2. University of St. Andrews, Centre for Biomolecular Sciences (United Kingdom)
Publication Date:
OSTI Identifier:
21090776
Resource Type:
Journal Article
Journal Name:
Crystallography Reports
Additional Journal Information:
Journal Volume: 52; Journal Issue: 5; Other Information: DOI: 10.1134/S1063774507050124; Copyright (c) 2007 Nauka/Interperiodica; Article Copyright (c) 2007 Pleiades Publishing, Inc; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1063-7745
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; COPPER; CRYSTAL STRUCTURE; ELECTRON TRANSFER; OXIDASES; SYNCHROTRON RADIATION; X-RAY DIFFRACTION

Citation Formats

Zhukova, Yu N, Zhukhlistova, N E, Lyashenko, A V, Morgunova, E Yu, Zaitsev, V N, and Mikhailov, A. M. Comparative analysis of spatial organization of laccases from Cerrena maxima and Coriolus zonatus. United States: N. p., 2007. Web. doi:10.1134/S1063774507050124.
Zhukova, Yu N, Zhukhlistova, N E, Lyashenko, A V, Morgunova, E Yu, Zaitsev, V N, & Mikhailov, A. M. Comparative analysis of spatial organization of laccases from Cerrena maxima and Coriolus zonatus. United States. https://doi.org/10.1134/S1063774507050124
Zhukova, Yu N, Zhukhlistova, N E, Lyashenko, A V, Morgunova, E Yu, Zaitsev, V N, and Mikhailov, A. M. Sat . "Comparative analysis of spatial organization of laccases from Cerrena maxima and Coriolus zonatus". United States. https://doi.org/10.1134/S1063774507050124.
@article{osti_21090776,
title = {Comparative analysis of spatial organization of laccases from Cerrena maxima and Coriolus zonatus},
author = {Zhukova, Yu N and Zhukhlistova, N E and Lyashenko, A V and Morgunova, E Yu and Zaitsev, V N and Mikhailov, A. M.},
abstractNote = {Laccase (oxygen oxidoreductase, EC 1.10.3.2) belongs to the multicopper oxidase family. The main function of this enzyme is to perform electron transfer from the oxidized substrate through the mononuclear copper-containing site T1 to the oxygen molecule bound to the site T3 in the trinuclear T2/T3 cluster. The structures of two new fungal laccases from C. maxima and C. zonatus were solved on the basis of synchrotron X-ray diffraction data. Both laccases show high structural homology with laccases from other sources. The role of the carbohydrate component of laccases in structure stabilization and formation of ordered protein crystals was demonstrated. In the structures of C. maxima and C. zonatus laccases, two water channels of functional importance were found and characterized. The structural results reported in the present study characterize one of the functional states of the enzyme fixed in the crystal structure.},
doi = {10.1134/S1063774507050124},
url = {https://www.osti.gov/biblio/21090776}, journal = {Crystallography Reports},
issn = {1063-7745},
number = 5,
volume = 52,
place = {United States},
year = {2007},
month = {9}
}