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U.S. Department of Energy
Office of Scientific and Technical Information

Short-term methods for estimating the chronic toxicity of effluents and receiving water to freshwater organisms. Third edition

Technical Report ·
OSTI ID:210828

This manual describes four short-term (four- to seven-day) methods for estimating the chronic toxicity of effluents and receiving waters to three freshwater species: The fathead minnow, Pimephales promelas, a daphnid, Ceriodaphnia dubia, and a green alga, Selenastrum capricornutum. The methods include single and multiple concentration static renewal and non-renewal toxicity tests for effluents and receiving waters. Also included are guidelines on laboratory safety, quality assurance, facilities, equipment and supplies; dilution water; effluent and receiving water sample collection, preservation, shipping, and holding; test conditions; toxicity test data analysis; report preparation; and organism culturing, holding, and handling.

Research Organization:
Environmental Protection Agency, Cincinnati, OH (United States). Environmental Monitoring Systems Lab.
OSTI ID:
210828
Report Number(s):
PB--96-141452/XAB; EPA--600/4-91/002
Country of Publication:
United States
Language:
English