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Title: Quantification of Horseradish Peroxidase Delivery into the Arterial Wall In Vivo as a Model of Local Drug Treatment: Comparison Between a Porous and a Gel-Coated Balloon Catheter

Abstract

Purpose: To quantify horseradish peroxidase (HRP) delivery into the arterial wall, as a model of local drug delivery, and to compare two different percutaneous delivery balloons. Methods: Perforated and hydrophilic hydrogel-coated balloon catheters were used to deliver HRP in aqueous solution into the wall of porcine iliac arteries in vivo. HRP solutions of 1 mg/ml were used together with both perforated and hydrophilic hydrogel-coated balloon catheters and 40 mg/ml HRP solutions were used with the hydrogel-coated balloon only. The amount of HRP deposited in the arterial wall was then determined photospectrometrically. Results: Using the 1 mg/ml HRP solution, the hydrogel-coated balloon absorbed 0.047 mg HRP into the coating. Treatment with this balloon resulted in a mean vessel wall concentration of 7.4 {mu}g HRP/g tissue {+-} 93% (standard deviation) (n 7). Treatment with the hydrogel-coated balloon that had absorbed 1.88 mg HRP into the coating (using the 40 mg/ml HRP solution) led to a mean vessel wall concentration of 69.5 {mu}g HRP/g tissue {+-} 74% (n = 7). Treatment with the perforated balloon using 1 mg/ml aqueous HRP solution led to a mean vessel wall concentration of 174 {mu}g/g {+-} 81% (n = 7). Differences between the hydrogel-coated and perforated balloonsmore » (1 mg/g solutions of HRP) and between hydrogel-coated balloons (0.047 mg vs 1.88 mg absorbed into the balloon coating) were significant (p < 0.05; two-sided Wilcoxon test). Conclusions: The use of a perforated balloon catheter allowed the delivery of a higher total amount of HRP compared with the hydrogel-coated balloon, but at the cost of a higher systemic HRP application. To deliver 174 {mu}g HRP per gram of vessel wall with the perforated balloon, 6.5 {+-} 1.5 mg HRP were lost into the arterial blood (delivery efficiency range = 0.2%-0.3%). With 0.047 mg HRP loaded into the coating of the hydrogel balloon, 7.4 {mu}g HRP could be applied to 1 g of vessel wall (delivery efficiency 1.7%), and with 1.88 mg HRP loaded into the coating of the hydrogel balloon, 69.5 {mu}g HRP could be applied per gram of vessel wall (delivery efficiency 0.6%)« less

Authors:
;  [1];  [2];  [1];  [2]; ;  [1]
  1. Department of Diagnostic Radiology, University of Technology Aachen, Pauwelsstrasse 30, D-52057 Aachen (Germany)
  2. Department of Physiology, University of Technology Aachen, Pauwelsstrasse 30, D-52057 Aachen (Germany)
Publication Date:
OSTI Identifier:
21080276
Resource Type:
Journal Article
Journal Name:
Cardiovascular and Interventional Radiology
Additional Journal Information:
Journal Volume: 22; Journal Issue: 5; Other Information: DOI: 10.1007/s002709900413; Copyright (c) 1999 Springer-Verlag New York Inc; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0174-1551
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AQUEOUS SOLUTIONS; ARTERIES; BLOOD; HYDROGELS; IN VIVO; PEROXIDASES; POROUS MATERIALS; VASCULAR DISEASES

Citation Formats

Dick, Armin, Kromen, Wolfgang, Juengling, Eberhard, Grosskortenhaus, Stephanie, Kammermeier, Helmut, Vorwerk, Dierk, and Guenther, Rolf W. Quantification of Horseradish Peroxidase Delivery into the Arterial Wall In Vivo as a Model of Local Drug Treatment: Comparison Between a Porous and a Gel-Coated Balloon Catheter. United States: N. p., 1999. Web. doi:10.1007/S002709900413.
Dick, Armin, Kromen, Wolfgang, Juengling, Eberhard, Grosskortenhaus, Stephanie, Kammermeier, Helmut, Vorwerk, Dierk, & Guenther, Rolf W. Quantification of Horseradish Peroxidase Delivery into the Arterial Wall In Vivo as a Model of Local Drug Treatment: Comparison Between a Porous and a Gel-Coated Balloon Catheter. United States. doi:10.1007/S002709900413.
Dick, Armin, Kromen, Wolfgang, Juengling, Eberhard, Grosskortenhaus, Stephanie, Kammermeier, Helmut, Vorwerk, Dierk, and Guenther, Rolf W. Wed . "Quantification of Horseradish Peroxidase Delivery into the Arterial Wall In Vivo as a Model of Local Drug Treatment: Comparison Between a Porous and a Gel-Coated Balloon Catheter". United States. doi:10.1007/S002709900413.
@article{osti_21080276,
title = {Quantification of Horseradish Peroxidase Delivery into the Arterial Wall In Vivo as a Model of Local Drug Treatment: Comparison Between a Porous and a Gel-Coated Balloon Catheter},
author = {Dick, Armin and Kromen, Wolfgang and Juengling, Eberhard and Grosskortenhaus, Stephanie and Kammermeier, Helmut and Vorwerk, Dierk and Guenther, Rolf W.},
abstractNote = {Purpose: To quantify horseradish peroxidase (HRP) delivery into the arterial wall, as a model of local drug delivery, and to compare two different percutaneous delivery balloons. Methods: Perforated and hydrophilic hydrogel-coated balloon catheters were used to deliver HRP in aqueous solution into the wall of porcine iliac arteries in vivo. HRP solutions of 1 mg/ml were used together with both perforated and hydrophilic hydrogel-coated balloon catheters and 40 mg/ml HRP solutions were used with the hydrogel-coated balloon only. The amount of HRP deposited in the arterial wall was then determined photospectrometrically. Results: Using the 1 mg/ml HRP solution, the hydrogel-coated balloon absorbed 0.047 mg HRP into the coating. Treatment with this balloon resulted in a mean vessel wall concentration of 7.4 {mu}g HRP/g tissue {+-} 93% (standard deviation) (n 7). Treatment with the hydrogel-coated balloon that had absorbed 1.88 mg HRP into the coating (using the 40 mg/ml HRP solution) led to a mean vessel wall concentration of 69.5 {mu}g HRP/g tissue {+-} 74% (n = 7). Treatment with the perforated balloon using 1 mg/ml aqueous HRP solution led to a mean vessel wall concentration of 174 {mu}g/g {+-} 81% (n = 7). Differences between the hydrogel-coated and perforated balloons (1 mg/g solutions of HRP) and between hydrogel-coated balloons (0.047 mg vs 1.88 mg absorbed into the balloon coating) were significant (p < 0.05; two-sided Wilcoxon test). Conclusions: The use of a perforated balloon catheter allowed the delivery of a higher total amount of HRP compared with the hydrogel-coated balloon, but at the cost of a higher systemic HRP application. To deliver 174 {mu}g HRP per gram of vessel wall with the perforated balloon, 6.5 {+-} 1.5 mg HRP were lost into the arterial blood (delivery efficiency range = 0.2%-0.3%). With 0.047 mg HRP loaded into the coating of the hydrogel balloon, 7.4 {mu}g HRP could be applied to 1 g of vessel wall (delivery efficiency 1.7%), and with 1.88 mg HRP loaded into the coating of the hydrogel balloon, 69.5 {mu}g HRP could be applied per gram of vessel wall (delivery efficiency 0.6%)},
doi = {10.1007/S002709900413},
journal = {Cardiovascular and Interventional Radiology},
issn = {0174-1551},
number = 5,
volume = 22,
place = {United States},
year = {1999},
month = {9}
}