In vivo immunomodulatory effects of Antrodia camphorata polysaccharides in a T1/T2 doubly transgenic mouse model for inhibiting infection of Schistosoma mansoni

Journal Article · Sat Mar 01 04:00:00 EST 2008 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2007.10.023· OSTI ID:21077944

Cheng, P -C ^[1]; Hsu, C -Y ^[2]; Chen, C -C ^[3]; Lee, K -M ^[1]

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1. Institute of Tropical Medicine, National Yang-Ming University, Taipei, Taiwan (China)
2. Institute of Molecular and Cellular Biology, National Tsing-Hua University, Hsinchu, Taiwan (China)
3. Biotechnology Center, Grape King Inc., Chungli, Taiwan (China)

Antrodia camphorata (A. camphorata) is a fungus commonly used for treatment of viral hepatitis and cancer in Chinese folk medicine. Extract of A. camphorate is reported to possess anti-inflammatory, antihepatitis B virus and anticancer activities. In this study, we tested the in vivo effects of polysaccharides derived from A. camphorata (AC-PS) on immune function by detection of cytokine expression and evaluation of the immune phenotype in a T1/T2 doubly transgenic mouse model. The protective effect of AC-PS in mice was tested by infection with Schistosoma mansoni. The induction of large amounts of IFN-{gamma}, IL-2 and TNF-a mRNA were detected after 2 and 4 weeks of oral AC-PS administration in BALB/c and C57BL/6 mice. In transgenic mice, 3 to 6 weeks of oral AC-PS administration increased the proportion of CD4{sup +} T cells and B cells within the spleen. More specifically, there was an increase of Th1 CD4{sup +} T cells and Be1 cells among spleen cells as observed by detection the of Type1/Type2 marker molecules. By using a disease model of parasitic infection, we found that AC-PS treatment inhibited infection with S. mansoni in BALB/C and C57BL/6 mice. AC-PS appears to influence the immune system of mice into developing Th1 responses and have potential for preventing infection with S. mansoni.

• OSTI ID: 21077944
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 2 Vol. 227; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DRUGS
EVALUATION
FUNGI
HEPATITIS
IN VIVO
INFLAMMATION
NEOPLASMS
PHENOTYPE
POLYSACCHARIDES
SCHISTOSOMA
SPLEEN
SPLEEN CELLS
TRANSGENIC MICE
VIRUSES