Real-time analysis of breath for carbon tetrachloride and its metabolites

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:210712

Kenny, D V; Callahan, P J ^[1]; Thrall, K D ^[2]

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1. Battelle Memorial Institute, Columbus, OH (United States)
2. Pacific Northwest Lab., Richland, WA (United States)

Real-time breath analysis offers a non-invasive method to detect exposure to toxic air pollutants. Breath measurements are useful in environmental exposure studies, and may provide evidence about previous long-term or repeated exposures in environments that are not easy to monitor. If breath samples are collected during or immediately following a short term exposure, breath measurements can be used to predict the peak exposure. Previous breath sampling methodologies have been to collect repeated 1-minute breath samples at 5 minute intervals. Although this method can aid in describing the rapid exponential emptying of the blood compartment that occurs following peak exposure, sampling 5 minute intervals still forces an approximation of the true shape of the clearance kinetics. The authors have developed a methodology to quantitively measure the concentration of exhaled volatile compounds in real-time using laboratory rats. Continuously monitoring breath for a parent toxicant and its metabolite(s) provides input into physiologically based pharmacokinetic (PBPK) models to describe the bio-kinetics of a chemical in the body. Real-time analyses are better than batch sampling methods because of the rapidly changing kinetics of elimination of some volatile chemicals from the body.

• OSTI ID: 210712
• Report Number(s): CONF-9505261--
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BREATH
CARBON TETRACHLORIDE
CHEMICAL ANALYSIS
KINETICS
MASS SPECTROSCOPY
METABOLITES
RATS