Molecular diagnostics via mass spectrometry of PCR-amplified DNA products
Conference
·
OSTI ID:210617
- Oak Ridge National Lab., TN (United States); and others
Identifying the presence of a specific DNA fragment is becoming increasingly critical to many applications in medical, clinical, forensic and other research laboratories. At present, regions of interest in DNA are amplified using the Polymerase Chain Reaction (PCR) or other reactions to produce fragments containing a specific number of nucleotide units that are diagnostic for the targeted genetic disease, person, or pathogen. These fragments are then typically analyzed by slab gel electrophoresis. Mass spectrometry has the potential of characterizing the DNA fragments faster and more confidently than chromatography-based methods. The authors have evaluated matrix assisted laser desorption (MALDI) time-of-flight (TOF) and electrospray (ES) quadrupole ion trap (QIT) mass spectrometry for the rapid analysis of PCR fragments.
- OSTI ID:
- 210617
- Report Number(s):
- CONF-9505261--
- Country of Publication:
- United States
- Language:
- English
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