Protease-catalyzed incorporation of {sup 18}O into protein fragments
Conference
·
OSTI ID:210612
- German Cancer Research Center, Heidelberg (Germany)
Normally, the preparation of an enzymatic digest containing a set of smaller peptide fragments is the first step in protein sequence analysis. Since proteolytic cleavage proceeds via incorporation of oxygen from water into the newly formed peptides, the reaction can be used to generate a set of carboxyl-{sup 18}O labeled peptides by using buffers containing H{sub 2}{sup 18}O. MALDI- and ESI-MS are the most powerful techniques for studying the incorporation of {sup 18}O in digest mixtures with or without LC separation of the peptide mixture.
- OSTI ID:
- 210612
- Report Number(s):
- CONF-9505261--
- Country of Publication:
- United States
- Language:
- English
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