Upgrade of IMCA-CAT Bending Magnet Beamline 17-BM for Macromolecular Crystallography at the Advanced Photon Source

Journal Article · Fri Jan 19 04:00:00 EST 2007 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.2436191· OSTI ID:21052649

Koshelev, I; Huang, R; Muir, J L; Battaile, K; Mulichak, A M; Keefe, L J ^[1]; Graber, T; Meron, M ^[2]; Lavender, W ^[3]

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1. IMCA-CAT, The Center for Advanced Radiation Sources, University of Chicago, Chicago, Illinois 60637 (United States)
2. ChemMatCARS, The Center for Advanced Radiation Sources, University of Chicago, Chicago, Illinois 60637 (United States)
3. Biological, Chemical, and Physical Sciences Department, Illinois Institute of Technology, Chicago, Illinois, 60616 (United States)

Pharmaceutical research depends on macromolecular crystallography as a tool in drug design and development. To solve the de novo three-dimensional atomic structure of a protein, it is essential to know the phases of the X-rays scattered by a protein crystal. Experimental phases can be obtained from multiwavelength anomalous dispersion (MAD) experiments. Dedicated to macromolecular crystallography, the IMCA-CAT bending magnet beamline at sector 17 of the Advanced Photon Source (APS) was upgraded to provide the energy resolution required to successfully perform synchrotron radiation-based MAD phasing of protein crystal structures. A collimating mirror was inserted into the beam path upstream of a double-crystal monochromator, thus increasing the monochromatic beam throughput in a particular bandwidth without sacrificing the energy resolution of the system. The beam is focused horizontally by a sagittally bent crystal and vertically by a cylindrically bent mirror, delivering a beam at the sample of 130 {mu}m (vertically) x 250 {mu}m (horizontally) FWHM. As a result of the upgrade, the beamline now operates with an energy range of 7.5x17.5 keV, delivers 8 x 10+11 photons/sec at 12.398 keV at the sample, and has an energy resolution of {delta}E/E = 1.45 x 10-4 at 10 keV, which is suitable for MAD experiments.

• OSTI ID: 21052649
• Journal Information: AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 879; ISSN APCPCS; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

43 PARTICLE ACCELERATORS
ADVANCED PHOTON SOURCE
BEAM BENDING MAGNETS
BEAM PRODUCTION
CRYSTAL STRUCTURE
CRYSTALS
DESIGN
ENERGY RESOLUTION
KEV RANGE
MIRRORS
MONOCHROMATIC RADIATION
MONOCHROMATORS
PHOTON BEAMS
PROTEINS
SYNCHROTRON RADIATION
X RADIATION
X-RAY DIFFRACTION