Optical Tweezers for Sample Fixing in Micro-Diffraction Experiments

Journal Article · Fri Jan 19 00:00:00 EST 2007 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.2436299· OSTI ID:21049260

Amenitsch, H; Rappolt, M; Sartori, B; Laggner, P ^[1]; Cojoc, D; Ferrari, E; Garbin, V; Di Fabrizio, E ^[2]; Burghammer, M; Riekel, Ch ^[3]

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1. Institute of Biophysics and X-ray Structure Research, Austrian Academy of Sciences, Schmiedlstr. 6, 8042 Graz (Austria)
2. CNR-INFM, Lab TASC, Area di Ricerca, 34012 Basovizza (Italy)
3. ESRF, 6 rue Jules Horowitz, BP220, 38043 Grenoble Cedex (France)

In order to manipulate, characterize and measure the micro-diffraction of individual structural elements down to single phospholipid liposomes we have been using optical tweezers (OT) combined with an imaging microscope. We were able to install the OT system at the microfocus beamline ID13 at the ESRF and trap clusters of about 50 multi-lamellar liposomes (< 10 {mu}m large cluster). Further we have performed a scanning diffraction experiment with a 1 micrometer beam to demonstrate the fixing capabilities and to confirm the size of the liposome cluster by X-ray diffraction.

• OSTI ID: 21049260
• Journal Information: AIP Conference Proceedings, Vol. 879, Issue 1; Conference: 9. international conference on synchrotron radiation instrumentation, Daegu (Korea, Republic of), 28 May - 2 Jun 2006; Other Information: DOI: 10.1063/1.2436299; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
EUROPEAN SYNCHROTRON RADIATION FACILITY
LAYERS
LIPOSOMES
OPTICAL SYSTEMS
PHOSPHOLIPIDS
SOLID CLUSTERS
TRAPS
X-RAY DIFFRACTION