RelA/NF-{kappa}B transcription factor associates with {alpha}-actinin-4

Journal Article · Mon Mar 10 04:00:00 EDT 2008 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2007.12.001· OSTI ID:21045947

Petukhova, Olga A; Turoverova, Lidia V; Kropacheva, Irina V; Tentler, Dmitri G; Bolshakova, Anastasia V ^[1]; Podolskaya, Ekaterina P ^[2]; Magnusson, Karl-Eric ^[3]; Pinaev, George P ^[1]

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1. Department of Cell Cultures, Institute of Cytology, Tikhoretsky Ave. 4, St. Petersburg, 194064 (Russian Federation)
2. Laboratory of Environmental and Biomedical Mass Spectrometry, Institute for Analytical Instrumentation, St. Petersburg, 198103 (Russian Federation)
3. Division of Medical Microbiology, Department of Clinical and Experimental Medicine, Linkoeping University, Linkoeping, SE-58185 (Sweden)

The NF-{kappa}B/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-{kappa}B in the cytoplasm and the transport mechanism to the nucleus. We found that NF-{kappa}B is associated with the actin-binding protein {alpha}-actinin-4. NF-{kappa}B and {alpha}-actinin-4 co-localized along actin stress fibers and in membrane lamellae in A431 cells. After a 30-min stimulation with EGF or TNF-{alpha}, {alpha}-actinin-4 and p65 were found in the nucleus. Disruption of cytoskeleton by cytochalasin D prior to treatment with TNF-{alpha} led to increase of p65 nuclear translocation. Antibodies to p65 subunit of NF-{kappa}B co-immunoprecipitated {alpha}-actinin-4 from A431 cell lysates and nuclear extracts, but {alpha}-actinin-1 and {beta}-actin were not found in the precipitates. Affinity chromatography experiments displayed that p65 and p50 subunits of NF-{kappa}B can bind to matrix-bound chicken gizzard {alpha}-actinin. We suggest that the {alpha}-actinin-4 is important for the NF-{kappa}B nuclear translocation and its functions inside the nucleus.

• OSTI ID: 21045947
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 5 Vol. 314; ISSN 0014-4827; ISSN ECREAL
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
ANTIBODIES
CHICKENS
CHROMATOGRAPHY
CYTOPLASM
FIBERS
MICROTUBULES
PRECIPITATION
TRANSCRIPTION
TRANSCRIPTION FACTORS
TRANSLOCATION