PTHrP promotes malignancy of human oral cancer cell downstream of the EGFR signaling

Journal Article · Fri Apr 11 04:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.01.121· OSTI ID:21043698

Yamada, Tamaki ^[1]; Tsuda, Masumi ^[1]; Ohba, Yusuke ^[1]; Kawaguchi, Hideaki ^[1]; Totsuka, Yasunori ^[2]; Shindoh, Masanobu ^[3]

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1. Laboratory of Pathophysiology and Signal Transduction, Department of Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kita-ku, Sapporo 060-8638 (Japan)
2. Laboratory of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, N13W7, Kita-ku, Sapporo 060-8586 (Japan)
3. Division of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, N13W7, Kita-ku, Sapporo 060-8586 (Japan)

Parathyroid hormone-related protein (PTHrP) is detected in many aggressive tumors and involved in malignant conversion; however, the underlying mechanism remains obscure. Here, we identified PTHrP as a mediator of epidermal growth factor receptor (EGFR) signaling to promote the malignancies of oral cancers. PTHrP mRNA was abundantly expressed in most of the quiescent oral cancer cells, and was significantly upregulated by EGF stimulation via ERK and p38 MAPK. PTHrP silencing by RNA interference, as well as EGFR inhibitor AG1478 treatment, significantly suppressed cell proliferation, migration, and invasiveness. Furthermore, combined treatment of AG1478 and PTHrP knockdown achieved synergistic inhibition of malignant phenotypes. Recombinant PTHrP substantially promoted cell motility, and rescued the inhibition by PTHrP knockdown, suggesting the paracrine/autocrine function of PTHrP. These data indicate that PTHrP contributes to the malignancy of oral cancers downstream of EGFR signaling, and may thus provide a therapeutic target for oral cancer.

• OSTI ID: 21043698
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 368; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
GROWTH FACTORS
HORMONES
INHIBITION
NEOPLASMS
PHENOTYPE
RECEPTORS
RNA
STIMULATION