Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

An automated for simultaneous and continuous monitoring of oxygen uptake and carbon dioxide evolution in bioreactors

Conference ·
OSTI ID:210332
;  [1];  [2]
  1. Univ. of Cincinnati, OH (United States)
  2. Environmental Protection Agency, Cincinnati, OH (United States)
+ Show Author Affiliations
A precision respiratory system for continuous and simultaneous on-line measurement of both oxygen uptake rate and carbon dioxide evolution rate was developed to assess the primary and ultimate biodegradability of organics in aqueous, soil slurry and other types of bioreactors. Oxygen uptake was measured with a conventional respirometer and carbon dioxide evolution was quantified by continuously measuring the change of conductivity of barium hydroxide solution, placed in a holder inside the respirometer flask. Continuous stirring of the barium hydroxide solution ensured that the absorption of carbon dioxide was not the controlling rate. The respiratory system was tested using aqueous biodegradation of phenol and crude oil in a soil slurry reactor. Results showed that the system is capable of continuously and automatically measuring on-line the cumulative oxygen uptake and carbon dioxide evolution. Further, the respiratory quotient, which is the ratio of oxygen uptake to carbon dioxide evolution rate, is usually close to one, but less than one before the oxygen curve plateau. This means that primary degradation is very similar to ultimate degradation. After the oxygen curve plateau, the carbon dioxide evolution rate is larger than the oxygen uptake rate, primarily due to biomass decay.
OSTI ID:
210332
Report Number(s):
CONF-9509139--
Country of Publication:
United States
Language:
English

Similar Records

Biodegradation of Cellulose in Laboratory-Scale Bioreactors: Experimental and Numerical Studies
Journal Article · Sat Dec 14 23:00:00 EST 2019 · Journal of Polymers and the Environment · OSTI ID:22959261
Evaluation of the natural biodegradation of jet fuel JP-8 in various soils using respirometry. Master`s thesis
Technical Report · Thu Nov 30 23:00:00 EST 1995 · OSTI ID:285127
Pentachlorophenol mineralization in an immobilized soil bioreactor
Conference · Mon Dec 30 23:00:00 EST 1996 · OSTI ID:430586

Related Subjects

44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
54 ENVIRONMENTAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIODEGRADATION
CARBON DIOXIDE
DECONTAMINATION
MONITORING
ON-LINE MEASUREMENT SYSTEMS
ORGANIC WASTES
OXYGEN
PERFORMANCE TESTING
PETROLEUM
PHENOLS
REAL TIME SYSTEMS
SOILS