LIV-1 suppression inhibits HeLa cell invasion by targeting ERK1/2-Snail/Slug pathway

Journal Article · Fri Nov 09 04:00:00 EST 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.08.127· OSTI ID:21032961

Le, Zhao; Wei, Chen ^[1]; Taylor, Kathryn M ^[2]; Bin, Cai ^[1]

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1. Center for Laboratory Medicine, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an (China)
2. Tenovus Centre for Cancer Research, Welsh School of Pharmacy, Cardiff University (United Kingdom)

It was reported that expression of the estrogen-regulated zinc transporter LIV-1 was particularly high in human cervical cancer cell line HeLa. This result prompted us to study the role that LIV-1 played in human cervical cancer. The results of real-time PCR showed that LIV-1 mRNA was significantly higher in cervical cancer in situ than in normal tissues. RNAi mediated suppression of LIV-1 in HeLa cells significantly inhibited cell proliferation, colony formation, migration, and invasive ability, but had no effect on cell apoptosis. Furthermore, LIV-1 suppression is accompanied by down-regulation of p44/42 MAPK, phospho-p44/42 MAPK, Snail and Slug expression levels. Hence, our data provide the first evidence that LIV-1 mRNA is overexpressed in cervical cancer in situ and is involved in invasion of cervical cancer cells through targeting MAPK-mediated Snail and Slug expression.

• OSTI ID: 21032961
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 363; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
CARCINOMAS
CELL PROLIFERATION
COLONY FORMATION
ESTROGENS
HELA CELLS
POLYMERASE CHAIN REACTION
SNAILS
ZINC