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Title: Degradation of carbon tetrachloride in a semi-continuous packed-bed bioreactor

Abstract

Pseudomonas sp. KC degrades carbon tetrachloride (CT) when grown under iron limited conditions. A packed-bed bioreactor was constructed out of glass and Teflon components and contained glass beads as a packing material. Total void volume of the reactor, including the pump reservoir, was approximately 300 mL. Media was recirculated through the reactor with a Teflon diaphragm pump and contained nitrate as terminal electron acceptor. After inoculation with KC and biofilm growth, the pump reservoir was replaced with fresh media and 3.0 mg/L CT was introduced to the reactor. When the initial CT had been degraded, 10% of the reactor volume was replaced with new media and CT was reintroduced. This process was repeated a second time. The initial degradation rate of the reactor increased from 75 {mu}g CT L{sup -1} hr{sup -1} for the first feeding to 109 {mu}g L{sup -1} hr{sup -1} for the third. A second reactor was packed with a Celite (diatomaceous earth) biocarrier and inoculated in a similar manner to the glass bead reactor. Degradation of CT in this reactor appeared to be suppressed, possibly due to the availability of iron to the bacteria from the biocarrier. While these experiments indicate it should be possible tomore » design a bioreactor utilizing Pseudomonas sp. KC to degrade CT in contaminated water, the suppression of degradative activity by iron will require special precautions to be taken in reactor design.« less

Authors:
; ;  [1]
  1. Univ. of Idaho, Moscow, ID (United States)
Publication Date:
OSTI Identifier:
210209
Report Number(s):
CONF-9509139-
TRN: 95:008324-0049
Resource Type:
Conference
Resource Relation:
Conference: 7. ACS special symposium: emerging technologies in hazardous waste management, Atlanta, GA (United States), 17-20 Sep 1995; Other Information: PBD: 1995; Related Information: Is Part Of Emerging technologies in hazardous waste management VII; Tedder, D.W. [ed.]; PB: 1352 p.
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; CARBON TETRACHLORIDE; DECHLORINATION; BIODEGRADATION; ORGANIC WASTES; WASTE PROCESSING; BIOREACTORS; PACKINGS; PSEUDOMONAS; DIATOMACEOUS EARTH; GLASS

Citation Formats

Levinson, W E, Korus, R A, and Crawford, R L. Degradation of carbon tetrachloride in a semi-continuous packed-bed bioreactor. United States: N. p., 1995. Web.
Levinson, W E, Korus, R A, & Crawford, R L. Degradation of carbon tetrachloride in a semi-continuous packed-bed bioreactor. United States.
Levinson, W E, Korus, R A, and Crawford, R L. Sun . "Degradation of carbon tetrachloride in a semi-continuous packed-bed bioreactor". United States.
@article{osti_210209,
title = {Degradation of carbon tetrachloride in a semi-continuous packed-bed bioreactor},
author = {Levinson, W E and Korus, R A and Crawford, R L},
abstractNote = {Pseudomonas sp. KC degrades carbon tetrachloride (CT) when grown under iron limited conditions. A packed-bed bioreactor was constructed out of glass and Teflon components and contained glass beads as a packing material. Total void volume of the reactor, including the pump reservoir, was approximately 300 mL. Media was recirculated through the reactor with a Teflon diaphragm pump and contained nitrate as terminal electron acceptor. After inoculation with KC and biofilm growth, the pump reservoir was replaced with fresh media and 3.0 mg/L CT was introduced to the reactor. When the initial CT had been degraded, 10% of the reactor volume was replaced with new media and CT was reintroduced. This process was repeated a second time. The initial degradation rate of the reactor increased from 75 {mu}g CT L{sup -1} hr{sup -1} for the first feeding to 109 {mu}g L{sup -1} hr{sup -1} for the third. A second reactor was packed with a Celite (diatomaceous earth) biocarrier and inoculated in a similar manner to the glass bead reactor. Degradation of CT in this reactor appeared to be suppressed, possibly due to the availability of iron to the bacteria from the biocarrier. While these experiments indicate it should be possible to design a bioreactor utilizing Pseudomonas sp. KC to degrade CT in contaminated water, the suppression of degradative activity by iron will require special precautions to be taken in reactor design.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

Conference:
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