Hop/STI1 modulates retinal proliferation and cell death independent of PrP{sup C}
- Universidade Federal do Rio de Janeiro (UFRJ), Instituto de Biofisica da UFRJ, Centro de Ciencias da Saude, bloco G, G2-019 Cidade Universitaria, 21941-902, Rio de Janeiro (Brazil)
Hop/STI1 is a co-chaperone adaptor protein for Hsp70/Hsp90 complexes. Hop/STI1 is found extracellularly and modulates cell death and differentiation through interaction with the prion protein (PrP{sup C}). Here, we investigated the expression of hop/STI1 and its role upon cell proliferation and cell death in the developing retina. Hop/STI1 is more expressed in developing rat retina than in the mature tissue. Hop/STI1 blocks retinal cell death in the neuroblastic layer (NBL) in a PrP{sup C} dependent manner, but failed to protect ganglion cells against axotomy-induced cell death. An antibody raised against hop/STI1 ({alpha}-STI1) blocked both ganglion cell and NBL cell death independent of PrP{sup C}. cAMP/PKA, ERK, PI3K and PKC signaling pathways were not involved in these effects. Hop/STI1 treatment reduced proliferation, while {alpha}-STI1 increased proliferation in the developing retina, both independent of PrP{sup C}. We conclude that hop/STI1 can modulate both proliferation and cell death in the developing retina independent of PrP{sup C}.
- OSTI ID:
- 20991547
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 361, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.07.038; PII: S0006-291X(07)01515-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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