Identification and characterization of a novel protein ISOC2 that interacts with p16{sup INK4a}

Journal Article · Fri Sep 21 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.06.181· OSTI ID:20991535

Huang, Xiaoyi ^[1]; Shi, Zhongcheng ^[1]; Wang, Wei ^[1]; Bai, Jing ^[1]; Chen, Zhenghua ^[2]; Xu, Jiujin ^[3]; Zhang, Dekai ^[4]; Fu, Songbin ^[1]

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1. Department of Medical Genetics, Harbin Medical University, Baojian Road, Harbin 150081 (China)
2. Post-doctoral Station Beijing Branch of Gansu Yasheng Salt Industrial (Group) Co. Ltd., Beijing (China)
3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing (China)
4. Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A and M University System Health Sciences Center, Houston, TX 77030 (United States)

p16{sup INK4a} is a multiple tumor suppressor, playing an important role in proliferation and tumorigenesis. To screen the p16{sup INK4a}-associated proteins, we performed a yeast two-hybrid assay and identified a novel protein isochorismatase domain containing 2 (ISOC2). ISOC2 conserves in different species, and encodes 205 and 210 amino acids in human and mouse, respectively. The expression of ISOC2 in mouse is universal but predominantly in uterus, stomach, and urinary tract system. Interaction between ISOC2 and p16{sup INK4a} was verified using in vitro pull-down assays and in vivo co-immunoprecipitation. Confocal microscopy studies using green and cyan fluorescent fusion proteins determined that ISOC2 co-localizes with p16{sup INK4a}. Over-expressed ISOC2 is able to inhibit p16{sup INK4a} in dose-dependent manner. Our data indicated that ISOC2 is a novel functional protein, which is able to bind and co-localize with a tumor suppressor gene p16{sup INK4a}. Over-expressed ISOC2 inhibits the expression of p16{sup INK4a}, suggesting that this novel gene may play a role during the tumor development by interacting with p16{sup INK4a}.

• OSTI ID: 20991535
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 2 Vol. 361; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AMINO ACIDS
CELL PROLIFERATION
GENES
HYBRIDIZATION
IN VITRO
IN VIVO
MICE
NEOPLASMS
PROTEINS
STOMACH
URINARY TRACT
UTERUS
YEASTS