Redox-linked conformational changes of a multiheme cytochrome from Geobacter sulfurreducens

Journal Article · Fri Aug 17 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.06.026· OSTI ID:20991497

Morgado, Leonor ^[1]; Bruix, Marta ^[2]; Londer, Yuri Y ^[3]; Pokkuluri, P Raj ^[3]; Schiffer, Marianne ^[3]; Salgueiro, Carlos A ^[1]

---

1. Requimte - CQFB, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica (Portugal)
2. Departamento de Espectroscopia y Estructura Molecular, Instituto de Quimica-Fisica 'Rocasolano', CSIC, Serrano 119, 28006 Madrid (Spain)
3. Biosciences Division, Argonne National Laboratory, Argonne, IL 60439 (United States)

Multiheme c-type cytochromes from members of the Desulfovibrionacea and Geobactereacea families play crucial roles in the bioenergetics of these microorganisms. Thermodynamic studies using NMR and visible spectroscopic techniques on tetraheme cytochromes c {sub 3} isolated from Desulfovibrio spp. and more recently on a triheme cytochrome from Geobacter sulfurreducens showed that the properties of each redox centre are modulated by the neighbouring redox centres enabling these proteins to perform energy transduction and thus contributing to cellular energy conservation. Electron/proton transfer coupling relies on redox-linked conformational changes that were addressed for some multiheme cytochromes from the comparison of protein structure of fully reduced and fully oxidised forms. In this work, we identify for the first time in a multiheme cytochrome the simultaneous presence of two different conformations in solution. This was achieved by probing the different oxidation stages of a triheme cytochrome isolated from G. sulfurreducens using 2D-NMR techniques. The results presented here will be the foundations to evaluate the modulation of the redox centres properties by conformational changes that occur during the reoxidation of a multiheme protein.

• OSTI ID: 20991497
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 360; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CONFORMATIONAL CHANGES
COUPLING
CYTOCHROMES
DESULFOVIBRIO
ENERGY CONSERVATION
MODULATION
NUCLEAR MAGNETIC RESONANCE
OXIDATION
PROTEIN STRUCTURE