Effect of resveratrol on proliferation and differentiation of embryonic cardiomyoblasts

Journal Article · Fri Aug 17 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.06.025· OSTI ID:20991496

Leong, C -W ^[1]; Wong, C H ^[2]; Lao, S -C ^[1]; Leong, Emilia Conceicao ^[1]; Lao, Iok Fong ^[1]; Law, Patrick Tik Wan ^[2]; Fung, Kwok Pui ^[2]; Tsang, Kam Sze ^[3]; Waye, Mary Miu-Yee ^[2]; Tsui, Stephen Kwok-Wing ^[2]; Yitao, Wang ^[1]; Lee, Simon Ming-Yuen ^[4]

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1. Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao (China)
2. Department of Biochemistry, Chinese University of Hong Kong, Hong Kong (China)
3. Department of Anatomical and Cellular Pathology, Chinese University of Hong Kong, Hong Kong (China)
4. Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao (China) and Institute of Chinese Medicine, Chinese University of Hong Kong, Hong Kong (China)

Resveratrol (trans-3,5,4'-trihydroxystilbene), a polyphenolic compound found largely in the skins of red grapes, has been used as a nutritional supplement or an investigational new drug for prevention of cardiovascular diseases. Previous reports showed that resveratrol had a protective effect against oxidative agent-induced cell injury. Our studies indicate that resveratrol plays a role in the differentiation of cardiomyoblasts. The cardiomyoblast cell line, H9c2, was exposed to 30-120 {mu}M resveratrol for up to 5 days. Resveratrol inhibits cardiomyoblast proliferation without causing cells injury. Moreover, resveratrol treatment modulated the differentiation of morphological characteristics including elongation and cell fusion in cardiomyoblasts. Proliferation and differentiation of H9c2 cells were further revealed by measurement of the mRNA expression of a cell cycle marker (CDK2), a differentiation marker (myogenin), and a contractile apparatus protein (MLC-2). Gene expression analysis revealed that resveratrol promoted entry into cell cycle arrest but extended the myogenic differentiation progress. These results have implications for the role of resveratrol in modulating cell cycle control and differentiation in cardiomyoblasts.

• OSTI ID: 20991496
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 360; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CARDIOVASCULAR DISEASES
CELL CYCLE
CELL PROLIFERATION
ELONGATION
GRAPES
INJURIES
OXIDATION
PROTEINS
SKIN