PPAR{gamma} activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide

Journal Article · Fri Aug 10 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.06.006· OSTI ID:20991485

Heo, Kyung-Sun ^[1]; Kim, Dong-Uk ^[1]; Ryoo, Sungwoo ^[1]; Nam, Miyoung ^[1]; Baek, Seung Tae ^[1]; Kim, Lila ^[1]; Park, Song-Kyu ^[2]; Myung, Chang-Seon ^[3]; Hoe, Kwang-Lae ^[1]

---

1. Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon (Korea, Republic of)
2. Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon (Korea, Republic of)
3. Department of Pharmacy, Chungnam National University, Yuseong, Daejeon (Korea, Republic of)

Native LDL would be a mitogenic and chemotactic stimulus of VSMC proliferation and differentiation in the atherosclerotic lesion where endothelial disruption occurred. In previous studies, our group investigated the molecular mechanisms by which LDL induces IL-8 production and by which PPAR{alpha} activation abolishes LDL effects in human aortic SMCs (hAoSMCs). Herein is the first report of PPAR{gamma} activation by troglitazone (TG) exerting its inhibitory effects on LDL-induced cell proliferation via generation not of H{sub 2}O{sub 2}, but of O2?-, and the subsequent activation of Erk1/2 in hAoSMCs. Moreover, in this study TG abolished the LDL-accelerated G{sub 1}-S progression to control levels via down-regulation of active cyclinD1/CDK4 and cyclinE/CDK2 complexes and up-regulation of p21{sup Cip1} expression. TG exerted its anti-proliferative effects through the up-regulation of basal superoxide dismutase (SOD) expression. This data suggests that the regulation of O2?- is located at the crossroads between LDL signaling and cell proliferation.

• OSTI ID: 20991485
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 359, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2007.06.006; PII: S0006-291X(07)01231-4; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ARTERIOSCLEROSIS
CELL PROLIFERATION
GENE REGULATION
HYDROGEN PEROXIDE
LIPOPROTEINS
MUSCLES
SUPEROXIDE DISMUTASE