Effects of Wnt-10b on hair shaft growth in hair follicle cultures

Journal Article · Fri Aug 03 04:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.05.135· OSTI ID:20991464

Ouji, Yukiteru ^[1]; Yoshikawa, Masahide ^[1]; Moriya, Kei ^[1]; Ishizaka, Shigeaki ^[1]

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1. Program in Tissue Engineering and Department of Parasitology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521 (Japan)

Wnts are deeply involved in the proliferation and differentiation of skin epithelial cells. We previously reported the differentiation of cultured primary skin epithelial cells toward hair shaft and inner root sheath (IRS) of the hair follicle via {beta}-catenin stabilization caused by Wnt-10b, however, the effects of Wnt-10b on cultured hair follicles have not been reported. In the present study, we examined the effects of Wnt-10b on shaft growth using organ cultures of whisker hair follicles in serum-free conditions. No hair shaft growth was observed in the absence of Wnt-10b, whereas its addition to the culture promoted elongation of the hair shaft, intensive incorporation of BrdU in matrix cells flanking the dermal papilla (DP), and {beta}-catenin stabilization in DP and IRS cells. These results suggest a promoting effect of Wnt-10b on hair shaft growth that is involved with stimulation of the DP via Wnt-10b/{beta}-catenin signalling, proliferation of matrix cells next to the DP, and differentiation of IRS cells by Wnt-10b.

• OSTI ID: 20991464
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 3 Vol. 359; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL GROWTH
CELL PROLIFERATION
ELONGATION
HAIR
HAIR FOLLICLES
STIMULATION
TISSUE CULTURES