SUMO modification modulates the transrepression activity of PLZF

Journal Article · Fri Jun 29 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.04.157· OSTI ID:20991410

Chao, T -T ^[1]; Chang, C -C ^[2]; Shih, H -M ^[3]

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1. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan (China)
2. Institute of Biomedical Sciences, Academia Sinica, 128, Sec. 2, Academia Road, Taipei 11529, Taiwan (China)
3. Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan (China) and Institute of Biomedical Sciences, Academia Sinica, 128, Sec. 2, Academia Road, Taipei 11529, Taiwan (China)

Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.

• OSTI ID: 20991410
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 358, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.04.157; PII: S0006-291X(07)00873-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
GENE REGULATION
GROWTH
INHIBITION
LYSINE
MODIFICATIONS
TRANSCRIPTION FACTORS