A new homolog of FocA transporters identified in cadmium-resistant Euglena gracilis
- IFR 141, Plateforme Transcriptome, Faculte de Pharmacie, Universite Paris-Sud, 5 rue Jean-Baptiste Clement, 92296 Chatenay-Malabry (France)
- Laboratoire de Biochimie et Biologie Cellulaire, Faculte de Pharmacie, Universite Paris-Sud, 5 rue Jean-Baptiste Clement, 92290 Chatenay-Malabry (France)
- FRE 2939, Institut Gustave Roussy (PR1), 39 rue Camille Desmoulins, 94805 Villejuif (France)
To better understand the cellular mechanism of stress resistance to various pollutants (cadmium, pentachlorophenol), we undertook a survey of the Euglena gracilis transcriptome by mRNA differential display and cDNA cloning. We performed a real-time RT-PCR analysis upon four selected genes. One of them significantly changed its expression level in response to stress treatments: B25 gene was overexpressed in Cd-resistant cells whereas it was down-regulated in PCP-adapted cells. By Race assays we obtained for B25 a 1093 bp cDNA. The deduced protein was identified as a bacterial formate/nitrite transporter (FocA) homolog and the gene was named EgFth. From all the data, we concluded that EgFth overexpression was related to chronic exposure to cadmium.
- OSTI ID:
- 20991408
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 358, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.04.145; PII: S0006-291X(07)00866-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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