Bone regeneration by implantation of adipose-derived stromal cells expressing BMP-2
- Department of Orthopaedic Surgery, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011 (China)
- Department of Orthopaedic Surgery, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011 (China) and Health and Science Center, SIBS CAS and SSMU, 225 South Chongqing Road, Shanghai 200025 (China)
- Health and Science Center, SIBS CAS and SSMU, 225 South Chongqing Road, Shanghai 200025 (China)
- Shanghai Institute of Biological Products, 1262 West Road Yanan, Shanghai 200052 (China)
In this study, we reported that the adipose-derived stromal cells (ADSCs) genetically modified by bone morphogenetic protein 2 (BMP-2) healed critical-sized canine ulnar bone defects. First, the osteogenic and adipogenic differentiation potential of the ADSCs derived from canine adipose tissue were demonstrated. And then the cells were modified by the BMP-2 gene and the expression and bone-induction ability of BMP-2 were identified. Finally, the cells modified by BMP-2 gene were applied to a {beta}-tricalcium phosphate (TCP) carrier and implanted into ulnar bone defects in the canine model. After 16 weeks, radiographic, histological, and histomorphometry analysis showed that ADSCs modified by BMP-2 gene produced a significant increase of newly formed bone area and healed or partly healed all of the bone defects. We conclude that ADSCs modified by the BMP-2 gene can enhance the repair of critical-sized bone defects in large animals.
- OSTI ID:
- 20991340
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 356, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2007.02.165; PII: S0006-291X(07)00454-8; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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