Characterization of two types of osteoclasts from human peripheral blood monocytes
- Department of Microbiology, Mie University Graduate School of Medicine (Japan) and Department of Orthopaedic Surgery, Mie University Graduate School of Medicine (Japan)
- Department of Orthopaedic Surgery, Mie University Graduate School of Medicine (Japan)
- Department of Public Health, Yamagata University Graduate School of Medicine (Japan)
- Department of Microbiology, Mie University Graduate School of Medicine (Japan)
The two osteoclastogenesis pathways, receptor activator nuclear factor (NF)-{kappa}B ligand (RANKL)-mediated and fusion regulatory protein-1 (FRP-1)-mediated osteoclastogenesis, have recently been reported. There were significant differences in differentiation and activation mechanisms between the two pathways. When monocytes were cultured with FRP-1 without adding M-CSF, essential for the RANKL system, TRAP-positive polykaryocyte formation occurred. FRP-1-mediated osteoclasts formed larger pits on mineralized calcium phosphate plates than RANKL+M-CSF-mediated osteoclasts did. Lacunae on dentin surfaces induced by FRP-1-mediated osteoclasts were inclined to be single and isolated. However, osteoclasts induced by RANKL+M-CSF made many connected pits on dentin surfaces as if they crawled on there. Interestingly, FRP-1 osteoclastogenesis was enhanced by M-CSF/IL-1{alpha}, while chemotactic behavior to the dentin slices was not effected. There were differences in pH and concentration of HCO3- at culture endpoint and in adherent feature to dentin surfaces. Our findings indicate there are two types of osteoclasts with distinct properties.
- OSTI ID:
- 20991321
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 356, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2007.02.118; PII: S0006-291X(07)00384-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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