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Title: RRM2 induces NF-{kappa}B-dependent MMP-9 activation and enhances cellular invasiveness

Abstract

Ribonucleotide reductase is a dimeric enzyme that catalyzes conversion of ribonucleotide 5'-diphosphates to their 2'-deoxynucleotide forms, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-triphosphates required for DNA synthesis. The ribonucleotide reductase M2 subunit (RRM2) is a determinant of malignant cellular behavior in a range of human cancers. We examined the effect of RRM2 overexpression on pancreatic adenocarcinoma cellular invasiveness and nuclear factor-{kappa}B (NF-{kappa}B) transcription factor activity. RRM2 overexpression increases pancreatic adenocarcinoma cellular invasiveness and MMP-9 expression in a NF-{kappa}B-dependent manner. RNA interference (RNAi)-mediated silencing of RRM2 expression attenuates cellular invasiveness and NF-{kappa}B activity. NF-{kappa}B is a key mediator of the invasive phenotypic changes induced by RRM2 overexpression.

Authors:
 [1];  [2]
  1. Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 (United States)
  2. Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115 (United States). E-mail: ewhang1@partners.org
Publication Date:
OSTI Identifier:
20979817
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 354; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.12.177; PII: S0006-291X(06)02856-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOSYNTHESIS; CARCINOMAS; DNA; ENZYMES; PANCREAS; RNA; TRANSCRIPTION FACTORS

Citation Formats

Duxbury, Mark S., and Whang, Edward E. RRM2 induces NF-{kappa}B-dependent MMP-9 activation and enhances cellular invasiveness. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2006.12.177.
Duxbury, Mark S., & Whang, Edward E. RRM2 induces NF-{kappa}B-dependent MMP-9 activation and enhances cellular invasiveness. United States. doi:10.1016/j.bbrc.2006.12.177.
Duxbury, Mark S., and Whang, Edward E. Fri . "RRM2 induces NF-{kappa}B-dependent MMP-9 activation and enhances cellular invasiveness". United States. doi:10.1016/j.bbrc.2006.12.177.
@article{osti_20979817,
title = {RRM2 induces NF-{kappa}B-dependent MMP-9 activation and enhances cellular invasiveness},
author = {Duxbury, Mark S. and Whang, Edward E.},
abstractNote = {Ribonucleotide reductase is a dimeric enzyme that catalyzes conversion of ribonucleotide 5'-diphosphates to their 2'-deoxynucleotide forms, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-triphosphates required for DNA synthesis. The ribonucleotide reductase M2 subunit (RRM2) is a determinant of malignant cellular behavior in a range of human cancers. We examined the effect of RRM2 overexpression on pancreatic adenocarcinoma cellular invasiveness and nuclear factor-{kappa}B (NF-{kappa}B) transcription factor activity. RRM2 overexpression increases pancreatic adenocarcinoma cellular invasiveness and MMP-9 expression in a NF-{kappa}B-dependent manner. RNA interference (RNAi)-mediated silencing of RRM2 expression attenuates cellular invasiveness and NF-{kappa}B activity. NF-{kappa}B is a key mediator of the invasive phenotypic changes induced by RRM2 overexpression.},
doi = {10.1016/j.bbrc.2006.12.177},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 354,
place = {United States},
year = {Fri Mar 02 00:00:00 EST 2007},
month = {Fri Mar 02 00:00:00 EST 2007}
}