Phytoestrogens levels determination in the cord blood from Malaysia rural and urban populations

Journal Article · Sun Jul 01 00:00:00 EDT 2007 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2007.03.014· OSTI ID:20976969

Mustafa, A M ^[1]; Malintan, N T ^[1]; Seelan, S ^[1]; Zhan, Z ^[2]; Mohamed, Z ^[1]; Hassan, J ^[3]; Pendek, R ^[4]; Hussain, R ^[5]; Ito, N ^[6]

---

1. Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur (Malaysia)
2. Shimadzu (Asia Pacific) Pte. Ltd., 16, Science Park Drive 01-01, The Pasteur, Singapore Science Park, 118227 Singapore (Singapore)
3. Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur (Malaysia)
4. Department of Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur (Malaysia)
5. Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur (Malaysia)
6. Asahi-Kasei Chemicals Corporation, Hibiya Mitsui Building, 1-2 Yurakucho 1-chome, Chiyoda-ku, Tokyo 100-8440 (Japan)

This study is a result of an analysis of free and conjugated phytoestrogens daidzein, genistein, daidzin, genistin and coumesterol in human cord blood plasma using LCMS. Cord blood was collected from urban and rural populations of Malaysia (n = 300) to establish a simple preliminary database on the levels of the analyzed compounds in the collected samples. The study also aimed to look at the levels of phytoestrogens in babies during birth as this may have a profound effect on the developmental process. The sample clean up was carried out by solid-phase extraction using C18 column and passed through DEAE sephadex gel before analysis by LCMS. The mean concentrations of total phytoestrogens were daidzein (1.4 {+-} 2.9 ng/ml), genistein (3.7 {+-} 2.8 ng/ml), daidzin (3.5 {+-} 3.1 ng/ml), genistin (19.5 {+-} 4.2 ng/ml) and coumesterol (3.3 {+-} 3.3 ng/ml). Distribution of phytoestrogen was found to be higher in samples collected from rural areas compared to that of urban areas.

• OSTI ID: 20976969
• Journal Information: Toxicology and Applied Pharmacology, Vol. 222, Issue 1; Other Information: DOI: 10.1016/j.taap.2007.03.014; PII: S0041-008X(07)00110-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BLOOD PLASMA
CARBON 18
EXTRACTION
GELS
MALAYSIA
RURAL AREAS
RURAL POPULATIONS
URBAN AREAS