Differential immune responses to albumin adducts of reactive intermediates of trichloroethene in MRL+/+ mice
Journal Article
·
· Toxicology and Applied Pharmacology
OSTI ID:20976919
- Department of Pathology, University of Texas Medical Branch, Galveston TX 77555-0609 (United States)
- Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555 (United States)
Trichloroethene (TCE) is an industrial degreasing solvent and widespread environmental contaminant. Exposure to TCE is associated with autoimmunity. The mode of action of TCE is via its oxidative metabolism, and most likely, immunotoxicity is mediated via haptenization of macromolecules and subsequent induction of immune responses. To better understand the role of protein haptenization through TCE metabolism, we immunized MRL+/+ mice with albumin adducts of various TCE reactive intermediates. Serum immunoglobulins and cytokine levels were measured to determine immune responses against haptenized albumin. We found antigen-specific IgG responses of the IgG subtypes IgG{sub 1}, IgG{sub 2a}, and IgG{sub 2b}, with IgG{sub 1} predominating. Serum levels of G-CSF were increased in immunized mice, suggesting macrophage activation. Liver histology revealed lymphocyte infiltration in the lobules and the portal area following immunization with formyl-albumin. Our findings suggest that proteins haptenized by metabolites of TCE may act as neo-antigens that can induce humoral immune responses and T cell-mediated hepatitis.
- OSTI ID:
- 20976919
- Journal Information:
- Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 3 Vol. 220; ISSN TXAPA9; ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
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