Metabolic differences between two dimethylthioarsenicals in rats
- Graduate School of Pharmaceutical Sciences, Chiba University, Chuo, Chiba 260-8675 (Japan)
Thioarsenicals are newly found arsenic metabolites in man and animals, and also in marine organisms. Dimethylmonothioarsinic acid (DMMTA{sup V}) and dimethyldithioarsinic acid (DMDTA{sup V}) are the only two thioarsenic metabolites detected in man and/or animals. However, their toxicological and biological significance is not known yet. The present study was performed to gain an insight into the significance of DMMTA{sup V} and DMDTA{sup V} in the metabolism of arsenic. The two thioarsenicals were synthesized chemically and injected intravenously into rats at the dose of 0.5 mg As/kg body weight. The distributions of arsenic in organs/tissues and body fluids were determined at 10 min and 12 h after the injection, and arsenic in liver and kidney supernatants, urine, plasma and red blood cell (RBC) lysates was subjected to speciation analysis by HPLC-ICP MS on a gel filtration GS 220 HQ column. Although both thioarsenicals are pentavalent arsenicals, they were distributed in organs/tissues and body fluids differently from the corresponding non-thiolated pentavalent arsenicals, and also from each other. Namely, DMMTA{sup V} was first found in organs/tissues at 10 min, and then redistributed and retained mostly in RBCs at 12 h, as in the case of trivalent dimethylarsinous acid (DMA{sup III}). On the other hand, although DMDTA{sup V} was also found in organs/tissues at 10 min, it had been efficiently excreted in urine in its intact form at 12 h. Thus, DMMTA{sup V} was unexpectedly distributed in and taken up by organs/tissues in a manner similar to DMA{sup III} rather than DMA{sup V}, whereas DMDTA{sup V} was distributed similarly to DMA{sup V} as expected, but was much more efficiently excreted in urine.
- OSTI ID:
- 20976850
- Journal Information:
- Toxicology and Applied Pharmacology, Vol. 218, Issue 2; Other Information: DOI: 10.1016/j.taap.2006.10.027; PII: S0041-008X(06)00407-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
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