Determination of Interbilayer and Transbilayer Lipid Transfers by Time-Resolved Small-Angle Neutron Scattering

Journal Article · Fri Jun 08 00:00:00 EDT 2007 · Physical Review Letters

DOI:https://doi.org/10.1103/PHYSREVLETT.98.238101· OSTI ID:20951474

Nakano, Minoru; Fukuda, Masakazu; Kudo, Takayuki; Handa, Tetsurou ^[1]; Endo, Hitoshi ^[2]

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1. Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501 (Japan)
2. Institute for Solid State Physics, The University of Tokyo, Tokai, 319-1106 (Japan)

We applied a time-resolved small-angle neutron scattering technique to the vesicle system of dimyristoylphosphatidylcholine for the first time to determine lipid kinetics. The observed kinetics could be explicitly represented by a simple model that includes two independent kinetic parameters, i.e., the rates of transbilayer and interbilayer exchange. This technique is perfectly suited for the determination of lipid exchange kinetics in equilibrium and applicable to evaluation of the activity of the factors relevant to lipid migration, such as translocase and lipid transfer proteins.

• OSTI ID: 20951474
• Journal Information: Physical Review Letters, Vol. 98, Issue 23; Other Information: DOI: 10.1103/PhysRevLett.98.238101; (c) 2007 The American Physical Society; Country of input: International Atomic Energy Agency (IAEA); ISSN 0031-9007
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
60 APPLIED LIFE SCIENCES
EQUILIBRIUM
EVALUATION
LIPIDS
NEUTRON DIFFRACTION
PROTEINS
SMALL ANGLE SCATTERING
TIME RESOLUTION