Label-free photoelectrochemical strategy for hairpin DNA hybridization detection on titanium dioxide electrode

Journal Article · Mon Dec 25 00:00:00 EST 2006 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.2420786· OSTI ID:20880205

Wu, Lu; Geng, Wang; Yan, Jin; Xin, Yao; Jianqiang, Hu; Jinghong, Li ^[1]

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1. Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084 (China)

A new photoelectrochemical strategy for hairpin DNA hybridization was devised, in which TiO{sub 2} served as the anchor and signal transducer, and no label or redox couples were required. Once the hybridization between hairpin DNA probe and target DNA occurred, the photocurrent would decrease, utilizing which the sequence of the target DNA could be identified. The sequence specificity experiment showed that one or more mismatches of DNA bases could be discriminated. This photoelectrochemical method would be a potential tool in DNA hybridization detection due to its great advantages: label-free, high sensitivity, specific recognition, low cost, and easy fabrication.

• OSTI ID: 20880205
• Journal Information: Applied Physics Letters, Vol. 89, Issue 26; Other Information: DOI: 10.1063/1.2420786; (c) 2006 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
BIOPHYSICS
DETECTION
DNA
DNA HYBRIDIZATION
ELECTRODES
PHOTOCONDUCTIVITY
SEMICONDUCTOR MATERIALS
SPECIFICITY
TITANIUM OXIDES