The ichthyotoxic alga Chattonella marina induces Na{sup +}, K{sup +}-ATPase, and CFTR proteins expression in fish gill chloride cells in vivo

Journal Article · Fri Feb 02 04:00:00 EST 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.11.126· OSTI ID:20857979

Tang, Janet Y.M. ^[1]; Wong, Chris K.C. ^[2]; Au, Doris W.T. ^[1]

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1. Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong (China)
2. Department of Biology, Baptist University of Hong Kong, 224 Waterloo Road, Kowloon (Hong Kong)

Our previous studies demonstrated that the ichthyotoxic Chattonella marina stimulated proliferation of branchial chloride cell (CC) and induced osmotic distress akin to hyperactive elimination of ions in fish (Rhabdosargus sarba). To ascertain the in vivo effects of C. marina on key CC ion transporters, the localization and expression of Na{sup +}, K{sup +}-ATPase (NKA) and cystic fibrosis transmembrane conductance regulator (CFTR) proteins in response to C. marina exposure were investigated, using a quantitative immunocytochemical approach. The polarized distributions of NKA ({alpha} subunit) and CFTR proteins in branchial CCs of R. sarba remained unchanged under C. marina exposure. However, significant inductions of these two ion-transporters were detected in CCs of fish after 6 h exposure. By real-time PCR, no significant changes in gill NKA and CFTR mRNA expressions were detected, suggesting a post-transcriptional pathway is likely involved in regulating the ion transporters abundance. This study is the first to demonstrate the in vivo effects of harmful algal toxin on NKA and CFTR protein expressions in gill transepithelial cells. Taken together, an augmentation of branchial CCs together with hyper-stimulation of NKA and CFTR in CCs attribute to the rapid development of osmotic distress in C. marina susceptible fish.

• OSTI ID: 20857979
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 353; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALGAE
CHLORIDES
FIBROSIS
GILLS
IN VIVO
MARINAS
POLYMERASE CHAIN REACTION
POTASSIUM IONS
PROTEINS
SODIUM IONS
TOXINS