Functionality of NGF-protected PC12 cells following exposure to 6-hydroxydopamine
Journal Article
·
· Biochemical and Biophysical Research Communications
- Department of Biochemistry, National University of Ireland, Galway (Ireland)
- National Centre for Biomedical Engineering and Science, National University of Ireland, Galway (Ireland)
- Department of Biochemistry, National University of Ireland, Galway (Ireland) and National Centre for Biomedical Engineering and Science, National University of Ireland, Galway (Ireland)
6-Hydroxydopamine (6-OHDA) is often used in models of Parkinson's disease since it can selectively target and kill dopaminergic cells of the substantia nigra. In this study, pre-treatment of PC12 cells with nerve growth factor (NGF) inhibited apoptosis and necrosis by 6-OHDA, including caspase activity and lactate dehydrogenase release. Notably, cells exposed to 6-OHDA in the presence of NGF were subsequently capable of proliferation (when replated without NGF), or neurite outgrowth (with continued presence of NGF). Following 7 days growth in the presence of NGF, expression of {beta}III tubulin and tyrosine hydroxylase and increased intracellular catecholamines was detectable in PC12 cells, features characteristic of functional dopaminergic neurons. NGF-pre-treated PC12 cells retained expression of {beta}III-tubulin and tyrosine hydroxylase, but not catecholamine content following 6-OHDA exposure. These data indicate that NGF-protected cells maintained some aspects of functionality and were subsequently capable of proliferation or differentiation.
- OSTI ID:
- 20857938
- Journal Information:
- Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 351; ISSN 0006-291X; ISSN BBRCA9
- Country of Publication:
- United States
- Language:
- English
Similar Records
Dopaminergic neurotoxicant 6-OHDA induces oxidative damage through proteolytic activation of PKC{delta} in cell culture and animal models of Parkinson's disease
Exposure of PC12 cells to NGF/ethanol results in accelerated differentiation and altered gene expression
Nerve growth factor-treated, neurite-bearing PC12 cells continue to synthesize DNA
Journal Article
·
Mon Nov 14 23:00:00 EST 2011
· Toxicology and Applied Pharmacology
·
OSTI ID:22212533
Exposure of PC12 cells to NGF/ethanol results in accelerated differentiation and altered gene expression
Conference
·
Sun Mar 10 23:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:5374490
Nerve growth factor-treated, neurite-bearing PC12 cells continue to synthesize DNA
Journal Article
·
Thu Jan 31 23:00:00 EST 1985
· J. Neurosci.; (United States)
·
OSTI ID:5554243