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Title: Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats-Correlation of HSP72 expression with mucosal protection

Abstract

Background and aim: The real mechanism of adaptive cytoprotection in the gastric mucosa is not well established. In the present study, we investigated the effect of acid suppressing agents on a 72-kDa heat shock protein (HSP72) expression, which is known as endogenous cytoprotective factor, in the gastric mucosa. Also, the association of gastric mucosal protective function against HCl-challenge was compared between HSP72-induced and -reduced group. Materials and methods: Expression of HSP72 was measured by Western blotting in the gastric mucosa before and after administration of famotidine or omeprazole. The gastric mucosal protective function against 0.6 N HCl was compared between control group and HSP72-reduced group. Also, the effect of increased expression of gastric HSP72 by additional administration of zinc sulfate or zinc L-carnosine, which is known as HSP72-inducer, on mucosal protective function was studied. Results: HSP72 expression in the gastric mucosa was reduced by acid suppressing agents. The lowest expression level of HSP72 was observed 12 h (famotidine, H2-receptor antagonist) or 48 h (omeprazole, proton pump inhibitor) after administration. The gastric mucosal protective ability against 0.6 N HCl was also reduced when HSP72 expression was decreased by famotidine or omeprazole. This phenomenon was reversed by HSP72 induction by additional administrationmore » of zinc derivatives. Conclusion: Our results might indicate that the expression of HSP72 in the gastric mucosa is physiologically regulated by gastric acid, and that HSP72 induction could be important in view of mucosal protection especially when HSP72 expression is reduced by administration of acid suppressing agents such as proton pump inhibitor or H2 receptor antagonist.« less

Authors:
 [1];  [2];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [3];  [1]
  1. Department of Internal Medicine and Gastroenterology, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543 (Japan)
  2. Department of Internal Medicine and Gastroenterology, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543 (Japan). E-mail: otaka@med.akita-u.ac.jp
  3. Department of Material-process Engineering and Applied Chemistry for Environment Akita University Faculty of Engineering and Resource Science, 1-1 Tegata Gakuen Town, Akita City 010-8502 (Japan)
Publication Date:
OSTI Identifier:
20854524
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 349; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2006.08.088; PII: S0006-291X(06)01877-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GASTRIC ACID; HEAT-SHOCK PROTEINS; HYDROCHLORIC ACID; MUCOUS MEMBRANES; PROTONS; RATS; RECEPTORS; ZINC; ZINC SULFATES

Citation Formats

Wada, Isao, Otaka, Michiro, Jin, Mario, Odashima, Masaru, Komatsu, Koga, Konishi, Noriaki, Matsuhashi, Tamotsu, Horikawa, Youhei, Ohba, Reina, Itoh, Hideaki, and Watanabe, Sumio. Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats-Correlation of HSP72 expression with mucosal protection. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.08.088.
Wada, Isao, Otaka, Michiro, Jin, Mario, Odashima, Masaru, Komatsu, Koga, Konishi, Noriaki, Matsuhashi, Tamotsu, Horikawa, Youhei, Ohba, Reina, Itoh, Hideaki, & Watanabe, Sumio. Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats-Correlation of HSP72 expression with mucosal protection. United States. doi:10.1016/j.bbrc.2006.08.088.
Wada, Isao, Otaka, Michiro, Jin, Mario, Odashima, Masaru, Komatsu, Koga, Konishi, Noriaki, Matsuhashi, Tamotsu, Horikawa, Youhei, Ohba, Reina, Itoh, Hideaki, and Watanabe, Sumio. Fri . "Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats-Correlation of HSP72 expression with mucosal protection". United States. doi:10.1016/j.bbrc.2006.08.088.
@article{osti_20854524,
title = {Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats-Correlation of HSP72 expression with mucosal protection},
author = {Wada, Isao and Otaka, Michiro and Jin, Mario and Odashima, Masaru and Komatsu, Koga and Konishi, Noriaki and Matsuhashi, Tamotsu and Horikawa, Youhei and Ohba, Reina and Itoh, Hideaki and Watanabe, Sumio},
abstractNote = {Background and aim: The real mechanism of adaptive cytoprotection in the gastric mucosa is not well established. In the present study, we investigated the effect of acid suppressing agents on a 72-kDa heat shock protein (HSP72) expression, which is known as endogenous cytoprotective factor, in the gastric mucosa. Also, the association of gastric mucosal protective function against HCl-challenge was compared between HSP72-induced and -reduced group. Materials and methods: Expression of HSP72 was measured by Western blotting in the gastric mucosa before and after administration of famotidine or omeprazole. The gastric mucosal protective function against 0.6 N HCl was compared between control group and HSP72-reduced group. Also, the effect of increased expression of gastric HSP72 by additional administration of zinc sulfate or zinc L-carnosine, which is known as HSP72-inducer, on mucosal protective function was studied. Results: HSP72 expression in the gastric mucosa was reduced by acid suppressing agents. The lowest expression level of HSP72 was observed 12 h (famotidine, H2-receptor antagonist) or 48 h (omeprazole, proton pump inhibitor) after administration. The gastric mucosal protective ability against 0.6 N HCl was also reduced when HSP72 expression was decreased by famotidine or omeprazole. This phenomenon was reversed by HSP72 induction by additional administration of zinc derivatives. Conclusion: Our results might indicate that the expression of HSP72 in the gastric mucosa is physiologically regulated by gastric acid, and that HSP72 induction could be important in view of mucosal protection especially when HSP72 expression is reduced by administration of acid suppressing agents such as proton pump inhibitor or H2 receptor antagonist.},
doi = {10.1016/j.bbrc.2006.08.088},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 349,
place = {United States},
year = {2006},
month = {10}
}