An essential GT motif in the lamin A promoter mediates activation by CREB-binding protein
- Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, Andhra Pradesh (India)
Lamin A is an important component of nuclear architecture in mammalian cells. Mutations in the human lamin A gene lead to highly degenerative disorders that affect specific tissues. In studies directed towards understanding the mode of regulation of the lamin A promoter, we have identified an essential GT motif at -55 position by reporter gene assays and mutational analysis. Binding of this sequence to Sp transcription factors has been observed in electrophoretic mobility shift assays and by chromatin immunoprecipitation studies. Further functional analysis by co-expression of recombinant proteins and ChIP assays has shown an important regulatory role for CREB-binding protein in promoter activation, which is mediated by the GT motif.
- OSTI ID:
- 20854492
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 348, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.07.171; PII: S0006-291X(06)01749-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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