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Title: The porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression

Abstract

The porcine lymphotropic herpesviruses (PLHV) are discussed as possible risk factors in xenotransplantation because of the high prevalence of PLHV-1, PLHV-2 and PLHV-3 in pig populations world-wide and the fact that PLHV-1 has been found to be associated with porcine post-transplant lymphoproliferative disease. To provide structural and functional knowledge on the PLHV immediate-early (IE) transactivator genes, the central regions of the PLHV genomes were characterized by genome walking, sequence and splicing analysis. Three spliced genes were identified (ORF50, ORFA6/BZLF1{sub h}, ORF57) encoding putative IE transactivators, homologous to (i) ORF50 and BRLF1/Rta (ii) K8/K-bZIP and BZLF1/Zta and (iii) ORF57 and BMLF1 of HHV-8 and EBV, respectively. Expressed as myc-tag or HA-tag fusion proteins, they were located to the cellular nucleus. In reporter gene assays, several PLHV-promoters were mainly activated by PLHV-1 ORF50, to a lower level by PLHV-1 ORFA6/BZLF1{sub h} and not by PLHV-1 ORF57. However, the ORF57-encoded protein acted synergistically on ORF50-mediated activation.

Authors:
 [1];  [2];  [1];  [1];  [1];  [1];  [1]
  1. Robert Koch-Institut, P14 Molekulare Genetik und Epidemiologie von Herpesviren, Nordufer 20, 13353 Berlin (Germany)
  2. Robert Koch-Institut, P14 Molekulare Genetik und Epidemiologie von Herpesviren, Nordufer 20, 13353 Berlin (Germany). E-mail: ehlersb@rki.de
Publication Date:
OSTI Identifier:
20850586
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 357; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2006.08.008; PII: S0042-6822(06)00544-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; PROMOTERS; PROTEINS; RISK ASSESSMENT; SWINE; TRANSPLANTS

Citation Formats

Lindner, I., Ehlers, B., Noack, S., Dural, G., Yasmum, N., Bauer, C., and Goltz, M. The porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression. United States: N. p., 2007. Web. doi:10.1016/j.virol.2006.08.008.
Lindner, I., Ehlers, B., Noack, S., Dural, G., Yasmum, N., Bauer, C., & Goltz, M. The porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression. United States. doi:10.1016/j.virol.2006.08.008.
Lindner, I., Ehlers, B., Noack, S., Dural, G., Yasmum, N., Bauer, C., and Goltz, M. Sat . "The porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression". United States. doi:10.1016/j.virol.2006.08.008.
@article{osti_20850586,
title = {The porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression},
author = {Lindner, I. and Ehlers, B. and Noack, S. and Dural, G. and Yasmum, N. and Bauer, C. and Goltz, M.},
abstractNote = {The porcine lymphotropic herpesviruses (PLHV) are discussed as possible risk factors in xenotransplantation because of the high prevalence of PLHV-1, PLHV-2 and PLHV-3 in pig populations world-wide and the fact that PLHV-1 has been found to be associated with porcine post-transplant lymphoproliferative disease. To provide structural and functional knowledge on the PLHV immediate-early (IE) transactivator genes, the central regions of the PLHV genomes were characterized by genome walking, sequence and splicing analysis. Three spliced genes were identified (ORF50, ORFA6/BZLF1{sub h}, ORF57) encoding putative IE transactivators, homologous to (i) ORF50 and BRLF1/Rta (ii) K8/K-bZIP and BZLF1/Zta and (iii) ORF57 and BMLF1 of HHV-8 and EBV, respectively. Expressed as myc-tag or HA-tag fusion proteins, they were located to the cellular nucleus. In reporter gene assays, several PLHV-promoters were mainly activated by PLHV-1 ORF50, to a lower level by PLHV-1 ORFA6/BZLF1{sub h} and not by PLHV-1 ORF57. However, the ORF57-encoded protein acted synergistically on ORF50-mediated activation.},
doi = {10.1016/j.virol.2006.08.008},
journal = {Virology},
number = 2,
volume = 357,
place = {United States},
year = {Sat Jan 20 00:00:00 EST 2007},
month = {Sat Jan 20 00:00:00 EST 2007}
}