skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Rabies virus glycoprotein as a carrier for anthrax protective antigen

Abstract

Live viral vectors expressing foreign antigens have shown great promise as vaccines against viral diseases. However, safety concerns remain a major problem regarding the use of even highly attenuated viral vectors. Using the rabies virus (RV) envelope protein as a carrier molecule, we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. In addition to the RV glycoprotein (G) transmembrane and cytoplasmic domains, a portion of the RV G ectodomain was required to express the chimeric RV G anthrax PA on the cell surface. The novel antigen was also efficiently incorporated into RV virions. Mice immunized with the inactivated recombinant RV virions exhibited seroconversion against both RV G and anthrax PA, and a second inoculation greatly increased these responses. These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems.

Authors:
 [1];  [1];  [2];  [3];  [1];  [1];  [3];  [4];  [4];  [1];  [5]
  1. Departments of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)
  2. Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029 (United States)
  3. Departments of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)
  4. (United States)
  5. Departments of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107 (United States) and Departments of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States). E-mail: matthias.schnell@jefferson.edu
Publication Date:
OSTI Identifier:
20850566
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 353; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2006.05.010; PII: S0042-6822(06)00331-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; BACILLUS; DISEASE VECTORS; GLYCOPROTEINS; INOCULATION; MICE; POLYPEPTIDES; RABIES; VACCINES; VIRUSES

Citation Formats

Smith, Mary Ellen, Koser, Martin, Xiao Sa, Siler, Catherine, McGettigan, James P., Calkins, Catherine, Pomerantz, Roger J., Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, Institute of Human Virology and Biodefense, Thomas Jefferson University, Philadelphia, PA 19107, Dietzschold, Bernhard, and Schnell, Matthias J. Rabies virus glycoprotein as a carrier for anthrax protective antigen. United States: N. p., 2006. Web. doi:10.1016/j.virol.2006.05.010.
Smith, Mary Ellen, Koser, Martin, Xiao Sa, Siler, Catherine, McGettigan, James P., Calkins, Catherine, Pomerantz, Roger J., Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, Institute of Human Virology and Biodefense, Thomas Jefferson University, Philadelphia, PA 19107, Dietzschold, Bernhard, & Schnell, Matthias J. Rabies virus glycoprotein as a carrier for anthrax protective antigen. United States. doi:10.1016/j.virol.2006.05.010.
Smith, Mary Ellen, Koser, Martin, Xiao Sa, Siler, Catherine, McGettigan, James P., Calkins, Catherine, Pomerantz, Roger J., Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, Institute of Human Virology and Biodefense, Thomas Jefferson University, Philadelphia, PA 19107, Dietzschold, Bernhard, and Schnell, Matthias J. Sat . "Rabies virus glycoprotein as a carrier for anthrax protective antigen". United States. doi:10.1016/j.virol.2006.05.010.
@article{osti_20850566,
title = {Rabies virus glycoprotein as a carrier for anthrax protective antigen},
author = {Smith, Mary Ellen and Koser, Martin and Xiao Sa and Siler, Catherine and McGettigan, James P. and Calkins, Catherine and Pomerantz, Roger J. and Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107 and Institute of Human Virology and Biodefense, Thomas Jefferson University, Philadelphia, PA 19107 and Dietzschold, Bernhard and Schnell, Matthias J.},
abstractNote = {Live viral vectors expressing foreign antigens have shown great promise as vaccines against viral diseases. However, safety concerns remain a major problem regarding the use of even highly attenuated viral vectors. Using the rabies virus (RV) envelope protein as a carrier molecule, we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. In addition to the RV glycoprotein (G) transmembrane and cytoplasmic domains, a portion of the RV G ectodomain was required to express the chimeric RV G anthrax PA on the cell surface. The novel antigen was also efficiently incorporated into RV virions. Mice immunized with the inactivated recombinant RV virions exhibited seroconversion against both RV G and anthrax PA, and a second inoculation greatly increased these responses. These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems.},
doi = {10.1016/j.virol.2006.05.010},
journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 353,
place = {United States},
year = {2006},
month = {9}
}