HPV16 E1E4 protein is phosphorylated by Cdk2/cyclin A and relocalizes this complex to the cytoplasm
- Division of Virology, MRC National Institute for Medical Research, London NW7 1AA (United Kingdom)
The human papillomavirus type 16 E1E4 protein is expressed abundantly in cells supporting viral DNA amplification, but its expression is lost during malignant progression. In cell culture, 16E1E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. Here, we show that 16E1E4 is also able to associate with cyclin A and Cdk2 during the G2 phase of the cell cycle. Only a weak association was apparent during S-phase, and progression through S-phase appeared unaffected. As with cyclin B1, the interaction of 16E1E4 with cyclin A is dependent on residues T22/T23 and results in the accumulation of cyclin A in the cytoplasm where it colocalizes with 16E1E4. 16E1E4 serine 32 was found to be phosphorylated by Cdk2/cyclin A. We hypothesize that the interaction of 16E1E4 with cyclin A may serve to increase the efficiency with which 16E1E4 is able to prevent mitotic entry.
- OSTI ID:
- 20850516
- Journal Information:
- Virology, Journal Name: Virology Journal Issue: 1 Vol. 349; ISSN VIRLAX; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
Similar Records
FOXM1c is activated by cyclin E/Cdk2, cyclin A/Cdk2, and cyclin A/Cdk1, but repressed by GSK-3{alpha}
Cyclin G2 is a centrosome-associated nucleocytoplasmic shuttling protein that influences microtubule stability and induces a p53-dependent cell cycle arrest
Cyclin E/Cdk2, P/CAF, and E1A regulate the transactivation of the c-myc promoter by FOXM1
Journal Article
·
Fri Sep 15 00:00:00 EDT 2006
· Biochemical and Biophysical Research Communications
·
OSTI ID:20854455
Cyclin G2 is a centrosome-associated nucleocytoplasmic shuttling protein that influences microtubule stability and induces a p53-dependent cell cycle arrest
Journal Article
·
Sat Dec 09 23:00:00 EST 2006
· Experimental Cell Research
·
OSTI ID:20858065
Cyclin E/Cdk2, P/CAF, and E1A regulate the transactivation of the c-myc promoter by FOXM1
Journal Article
·
Fri Mar 28 00:00:00 EDT 2008
· Biochemical and Biophysical Research Communications
·
OSTI ID:21043673