Paeonol attenuates TNBS-induced colitis by inhibiting NF-{kappa}B and STAT1 transactivation

Journal Article · Wed Nov 15 04:00:00 EST 2006 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2006.07.002· OSTI ID:20850476

Ishiguro, Kazuhiro ^[1]; Ando, Takafumi ^[2]; Maeda, Osamu ^[2]; Hasegawa, Motofusa ^[2]; Kadomatsu, Kenji ^[3]; Ohmiya, Naoki ^[2]; Niwa, Yasumasa ^[2]; Xavier, Ramnik ^[4]; Goto, Hidemi ^[2]

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1. Molecular Biology and Pathogenesis of Gastroenterology, School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi, 466-8550 (Japan)
2. Department of Gastroenterology, Graduate School of Medicine, Nagoya University, Aichi (Japan)
3. Department of Biochemistry, Graduate School of Medicine, Nagoya University, Aichi (Japan)
4. Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114 (United States)

Paeonol, a major phenolic component of Moutan Cortex, is known to have anti-inflammatory activity. However, the effect of Paeonol on colitis has not been evaluated and the molecular mechanism of its anti-inflammatory action remains unknown. The aim of this study was to determine if Paeonol enema attenuates trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. We also investigated the effects of Paeonol in colon cancer-derived CW-2 cells and T cell leukemia-derived Jurkat cells treated with tumor necrosis factor {alpha} (TNF{alpha}) and/or interferon {gamma} (IFN{gamma}), which play critical roles in TNBS-induced colitis. Paeonol enema attenuated TNBS-induced colitis judging by body weigh reduction, colon length and histological score. Myeloperoxidase activity and inducible nitric oxide synthase (iNOS) production in the colon were also reduced with Paeonol enema. In CW-2 cells, Paeonol inhibited iNOS protein and mRNA expression induced by costimulation of TNF{alpha} and IFN{gamma}. Furthermore, Paeonol reduced TNF{alpha}-induced NF-{kappa}B transactivation and IFN{gamma}-induced STAT1 transactivation in CW-2 cells and also in Jurkat cells. These findings suggest that Paeonol enema may be useful for the treatment of colitis.

• OSTI ID: 20850476
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 1 Vol. 217; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
INDIUM OXIDES
INFLAMMATION
INTERFERON
LARGE INTESTINE
LEUKEMIA
MICE
NITRIC OXIDE
SULFONIC ACIDS
TRANSCRIPTION FACTORS