2,3,7,8-Tetrachlorodibenzo-p-dioxin specifically reduces mRNA for the mineralization-related dentin sialophosphoprotein in cultured mouse embryonic molar teeth

Journal Article · Tue Oct 31 23:00:00 EST 2006 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2006.06.015· OSTI ID:20850461

Kiukkonen, Anu ^[1]; Sahlberg, Carin ^[2]; Lukinmaa, Pirjo-Liisa ^[3]; Alaluusua, Satu ^[2]; Peltonen, Eija ^[2]; Partanen, Anna-Maija ^[2]

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1. Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, Helsinki (Finland) and Department of Oral Pathology, Institute of Dentistry, University of Helsinki, Helsinki (Finland)
2. Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, Helsinki (Finland)
3. Department of Oral Pathology, Institute of Dentistry, University of Helsinki, Helsinki (Finland)

Previous studies show that the most toxic dioxin congener, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), interferes with mineralization of the dental matrices in developing mouse and rat teeth. Culture of mouse embryonic molar teeth with TCDD leads to the failure of enamel to be deposited and dentin to undergo mineralization. Lactationally exposed rats show defectively matured enamel and retardation of dentin mineralization. To see if the impaired mineralization is associated with changes in the expression of dentin sialophosphoprotein (Dspp), Bono1 and/or matrix metalloproteinase-20 (MMP-20), thought to be involved in mineralization of the dental hard tissues, we cultured mouse (NMRI) E18 mandibular molars for 3, 5 or 7 days and exposed them to 1 {mu}M TCDD after 2 days of culture. As detected by in situ hybridization of tissue sections, localization and intensity of Bono1 and MMP-20 expression showed no definite difference between the control and exposed tooth explants, suggesting that TCDD does not affect their expression. On the contrary, TCDD reduced or prevented the expression of Dspp in secretory odontoblasts and decreased it in presecretory ameloblasts. The results suggest that the retardation of dentin mineralization by TCDD in mouse molar teeth involves specific interference with Dspp expression.

• OSTI ID: 20850461
• Journal Information: Toxicology and Applied Pharmacology, Journal Name: Toxicology and Applied Pharmacology Journal Issue: 3 Vol. 216; ISSN TXAPA9; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DENTIN
DIOXIN
ENAMELS
FAILURES
IN-SITU HYBRIDIZATION
MICE
MINERALIZATION
RATS
TEETH