The sensitivity of human mesenchymal stem cells to ionizing radiation

Journal Article · Fri Sep 01 00:00:00 EDT 2006 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/j.ijrobp.2006.03.062· OSTI ID:20850078

Chen, M -F ^[1]; Lin, C -T ^[2]; Chen, W -C ^[1]; Yang, C -T ^[3]; Chen, C -C ^[3]; Liao, S -K ^[3]; Liu, J M ^[2]; Lu, C -H ^[3]; Lee, K -D ^[4]

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1. Department of Radiation Oncology, Chang Gung Memorial Hospital, Chiayi, Taiwan (China)
2. Division of Cancer Research, National Health Research Institutes, Taipei, Taiwan (China)
3. Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine and Chang Gung Institute of Technology, Tao-Yuan, Taiwan (China)
4. Graduate Institute of Clinical Medical Sciences, Chang Gung University College of Medicine and Chang Gung Institute of Technology, Tao-Yuan, Taiwan (China) and Department of Hematology and Oncology, Chang Gung Memorial Hospital, Chiayi, Taiwan (China)

Purpose: Recent studies have shown that mesenchymal stem cells (MSCs) obtained from bone marrow transplantation patients originate from the host. This clinical observation suggests that MSCs in their niches could be resistant to irradiation. However, the biologic responses of bone marrow MSCs to irradiation have rarely been described in the literature. Methods and Materials: In this study, human bone marrow-derived, clonally expanded MSCs were used to investigate their sensitivity to irradiation in vitro, and the cellular mechanisms that may facilitate resistance to irradiation. The human lung cancer cell line A549 and the breast cancer cell line HCC1937 were used as controls for radiosensitivity; the former line has been shown to be radioresistant and the latter radiosensitive. We then examined their in vitro biologic changes and sensitivities to radiation therapy. Results: Our results suggest that MSCs are characterized as resistant to irradiation. Several cellular mechanisms were demonstrated that may facilitate resistance to irradiation: ATM protein phosphorylation, activation of cell-cycle checkpoints, double-strand break repair by homologous recombination and nonhomologous end joining (NHEJ), and the antioxidant capacity for scavenging reactive oxygen species. Conclusions: As demonstrated, MSCs possess a better antioxidant reactive oxygen species-scavenging capacity and active double-strand break repair to facilitate their radioresistance. These findings provide a better understanding of radiation-induced biologic responses in MSCs and may lead to the development of better strategies for stem cell treatment and cancer therapy.

• OSTI ID: 20850078
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Vol. 66, Issue 1; Other Information: DOI: 10.1016/j.ijrobp.2006.03.062; PII: S0360-3016(06)00683-3; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ANTIOXIDANTS
BONE MARROW
CELL CYCLE
DNA REPAIR
IN VITRO
IONIZING RADIATIONS
IRRADIATION
LUNGS
MAMMARY GLANDS
NEOPLASMS
PATIENTS
PHOSPHORYLATION
RADIOSENSITIVITY
RADIOTHERAPY
STEM CELLS
STRAND BREAKS